Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes

	Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes
	Yu, LY; Lin, B; Zhang, ZL; Guo, LH
刊名	ACTA PHARMACOLOGICA SINICA
	2004
卷号	25 期号:6 页码:721-726
关键词	adenoviridae streptozocin insulin-dependent diabetes mellitus gene therapy nitric oxide glucose amylases reversed transcriptase polymerase chain reaction Western blotting
通讯作者	Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,lhguo@sunm.shcnc.ac.cn
英文摘要	AIM: To investigate the efficiency of transfer of A20 gene into pancreas against STZ-induced diabetes. METHODS: PVP-plasmid mixture was directly transferred into the pancreatic parenchyma 2 d before STZ injection. The uptake of plasmid pcDNA3-LacZ or pcDNA3-A20 was detected by PCR and the expression of LacZ was confirmed by histological analysis with X-gal. A20 expression in the pancreas of pcDNA3-A20 transgenic mice was measured by RT-PCR and Western blots. Urine amylase, NO generation, and histological examination were examined. RESULTS: Injection of PVP-plasmid mixture directly into the pancreatic parenchyma increased urine amylase concentration 16 h after operation and reversed it to nearly normal 36 h later. On d 33 LacZ expression could be found in spleen, duodenum, and islets. The development of diabetes was prevented by direct A20 gene transferring into the pancreas and A20-mediated protection was correlated with suppression of NO production. The insulitis was ameliorated in A20-treated mice. CONCLUSION: Injection of PVP-plasmid mixture directly into the pancreatic parenchyma led to target gene expression in islets. Direct transfer of A20 gene into the pancreas protected mice from STZ-induced diabetes.
学科主题	Chemistry; Pharmacology & Pharmacy
类目[WOS]	Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
关键词[WOS]	ZINC-FINGER PROTEIN ; MOUSE PANCREAS ; NITRIC-OXIDE ; APOPTOSIS ; CELLS ; ACTIVATION ; ADENOVIRUS ; DELIVERY ; MELLITUS ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000221833900003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2099]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yu, LY,Lin, B,Zhang, ZL,et al. Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes[J]. ACTA PHARMACOLOGICA SINICA,2004,25(6):721-726.
APA	Yu, LY,Lin, B,Zhang, ZL,&Guo, LH.(2004).Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes.ACTA PHARMACOLOGICA SINICA,25(6),721-726.
MLA	Yu, LY,et al."Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes".ACTA PHARMACOLOGICA SINICA 25.6(2004):721-726.