A stable upstream stem-loop structure enhances selection of the first 5 '-ORF-AUG as a main start codon for translation initiation of human ACATl mRNA

	A stable upstream stem-loop structure enhances selection of the first 5 '-ORF-AUG as a main start codon for translation initiation of human ACATl mRNA
	Yang, L; Chen, J; Chang, CCY; Yang, XY; Wang, ZZ; Chang, TY; Li, BL
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2004
卷号	36 期号:4 页码:259-268
关键词	ACATI start codon Kozak sequence stem-loop structure
通讯作者	Li, BL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,blli@sibs.ac.cn
英文摘要	Human ACAT 1 cDNA K1 was first cloned and functionally expressed in 1993. There are two adjacent in-frame AUG codons, AUG(1397-1399) and AUG(1415-1417), at 5'-terminus of the open reading frame (ORF, nt 1397-3049) of human ACAT 1 mRNA corresponding to cDNA K1. In current work, these two adjacent in-frame AUGs at 5'-terminus of the predicted ORF (5'-ORF-AUGs) as start codons for translation initiation of human ACAT1 mRNA were characterized in detail. Codon mutations indicated that both of these two adjacent 5'-ORF-AUGs can be selected as start codons but the first 5'-ORF-AUG(1397-1399) is a main start codon consistent with that of the predicted ORF of human ACAT1 mRNA. Further deletion and mutation analyses demonstrated that a stable upstream stem-loop structure enhanced the selection of the first 5'-ORF-AUG(1397-1399) as a main start codon, in addition to upstream nucleotide A in the -3 position, which is a key site of Kozak sequence. In addition, result of ACAT1 enzymatic activity assay showed no obvious difference between these two ACAT1 proteins respectively initiated from the two adjacent 5'-ORF-AUGs. This work showed that a stable upstream stem-loop structure could modulate the start codon selection during translation initiation of mRNAs that contain adjacent multi-5'-ORF-AUGs.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	A-CHOLESTEROL ACYLTRANSFERASE ; ACYL-COENZYME ; SECONDARY STRUCTURE ; EUKARYOTIC RIBOSOMES ; HUMAN LIVER ; PROTEIN ; COA ; RECOGNITION ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000221952000003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2075]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, L,Chen, J,Chang, CCY,et al. A stable upstream stem-loop structure enhances selection of the first 5 '-ORF-AUG as a main start codon for translation initiation of human ACATl mRNA[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(4):259-268.
APA	Yang, L.,Chen, J.,Chang, CCY.,Yang, XY.,Wang, ZZ.,...&Li, BL.(2004).A stable upstream stem-loop structure enhances selection of the first 5 '-ORF-AUG as a main start codon for translation initiation of human ACATl mRNA.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(4),259-268.
MLA	Yang, L,et al."A stable upstream stem-loop structure enhances selection of the first 5 '-ORF-AUG as a main start codon for translation initiation of human ACATl mRNA".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.4(2004):259-268.