Adipose tissue-derived stromal cells express neuronal phenotypes

	Adipose tissue-derived stromal cells express neuronal phenotypes
	Yang, LY; Liu, XM; Sun, B; Hui, GZ; Fei, J; Guo, LH
刊名	CHINESE MEDICAL JOURNAL
	2004
卷号	117 期号:3 页码:425-429
关键词	stromal cells adipose tissue neuron phenotype
通讯作者	Hui, GZ (reprint author), Suzhou Univ, Dept Neurosurg, Affiliated Hosp 1, Suzhou 215006, Peoples R China.,yangleeyee@sina.com
英文摘要	Background Adipose tissue-derived stromal cells (ADSCs) can be greatly expanded in vitro, and induced to differentiate into multiple mesenchymal cell types, including osteogenic, chondrogenic, myogenic, and adipogenic cells. This study was designed to investigate the possibility of ADSCs differentiating into neurons. Methods Adipose tissue from rats was digested with collagenase, and adherent stromal cells were cultured. A medium containing a low concentration of fetal bovine serum was adopted to induce the cells to differentiate. ADSCs were identified by immunocytochemistry, and semi-quantitative RT-PCR was applied to detect mRNA expression of neurofilament 1 (NF1) nestin, and neuron-specific enolase (NSE). Results Nestin-positive cells were found occasionally among ADSCs. ADSCs were found to express NSE mRNA and nestin mRNA, but not NF1 mRNA. ADSCs could differentiate into neuron-like cells in a medium composed of a low concentration of fetal bovine serum, and these differentiated cells displayed complicated neuron-like morphologies. Conclusions The data support the hypothesis that adipose tissue contains stem cells capable of differentiating into neurons. These stem cells can overcome their mesenchymal commitment, and may represent an alternative autologous stem cell source for CNS cell transplantation.
学科主题	General & Internal Medicine
类目[WOS]	Medicine, General & Internal
关键词[WOS]	MESENCHYMAL STEM-CELLS ; BONE-MARROW ; IN-VITRO ; NEURAL CELLS ; DIFFERENTIATE ; PROGENITORS ; BRAINS ; VIVO ; RAT
收录类别	SCI
语种	英语
WOS记录号	WOS:000220634700020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2059]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, LY,Liu, XM,Sun, B,et al. Adipose tissue-derived stromal cells express neuronal phenotypes[J]. CHINESE MEDICAL JOURNAL,2004,117(3):425-429.
APA	Yang, LY,Liu, XM,Sun, B,Hui, GZ,Fei, J,&Guo, LH.(2004).Adipose tissue-derived stromal cells express neuronal phenotypes.CHINESE MEDICAL JOURNAL,117(3),425-429.
MLA	Yang, LY,et al."Adipose tissue-derived stromal cells express neuronal phenotypes".CHINESE MEDICAL JOURNAL 117.3(2004):425-429.