Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells

	Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells
	Wang, L; Yang, GZ; Wu, XF
刊名	PROTEIN AND PEPTIDE LETTERS
	2004
卷号	11 期号:1 页码:63-69
关键词	BTMD-mPC6B Tn-5B1-4 insect cells
通讯作者	Wu, XF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xfwu@sumn.shcnc.ac.cn
英文摘要	we constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B protein and with enzymatic activity.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	SUBTILISIN-LIKE PROTEASE ; KEX2-LIKE PROCESSING ENDOPROTEASE ; PROHORMONE CONVERTASES ; YEAST KEX2 ; IDENTIFICATION ; CLEAVAGE ; ACTIVATION ; GENE ; FAMILY ; REGION
收录类别	SCI
语种	英语
WOS记录号	WOS:000220210400010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2018]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, L,Yang, GZ,Wu, XF. Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells[J]. PROTEIN AND PEPTIDE LETTERS,2004,11(1):63-69.
APA	Wang, L,Yang, GZ,&Wu, XF.(2004).Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells.PROTEIN AND PEPTIDE LETTERS,11(1),63-69.
MLA	Wang, L,et al."Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells".PROTEIN AND PEPTIDE LETTERS 11.1(2004):63-69.