beta-Catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells

	beta-Catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells
	Pan, WJ; Jia, YY; Wang, JY; Tao, DL; Gan, XQ; Tsiokas, L; Jing, NH; Wu, DQ; Li, L
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2005
卷号	102 期号:48 页码:17378-17383
关键词	Wnt
通讯作者	Li, L (reprint author), Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Farmington, CT 06030 USA.,dwu@neuron.uchc.edu ; lli@sibs.ac.cn
英文摘要	Wnt/beta-catenin signaling plays a critical role in embryonic myogenesis. Here we show that, in P19 embryonic carcinoma stem cells, Wnt/beta-catenin signaling initiates the myogenic process depends on beta-catenin-mediated relief of 1-mfa (inhibitor of MyoD Family a) suppression of myogenic regulatory factors (MRFs). We found that beta-catenin interacted with 1-mfa and that the interaction was enhanced by Wnt3a. In addition, we found that the interaction between beta-catenin and 1-mfa was able to attenuate the interaction of 1-mfa with MRFs, relieve 1-mfa-mediated suppression of the transcriptional activity and cytosolic sequestration of MRFs, and initiate myogenesis in a P1 9 myogenic model system that expresses exogenous myogenin. This work reveals a mechanism for the regulation of MRFs during myogenesis by elucidating beta-catenin-mediated, but lymphoid enhancing factor-1/T cell factor independent, mechanism in regulation of myogenic fate specification and differentiation of P19 mouse stem cells.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	WNT SIGNALING PATHWAY ; SKELETAL MYOGENESIS ; TRANSCRIPTION FACTORS ; MYOD FAMILY ; DEP DOMAIN ; ACTIVATION ; EXPRESSION ; GENE ; SPECIFICATION ; SOMITE
收录类别	SCI
语种	英语
WOS记录号	WOS:000233762000022
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1993]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Pan, WJ,Jia, YY,Wang, JY,et al. beta-Catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2005,102(48):17378-17383.
APA	Pan, WJ.,Jia, YY.,Wang, JY.,Tao, DL.,Gan, XQ.,...&Li, L.(2005).beta-Catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,102(48),17378-17383.
MLA	Pan, WJ,et al."beta-Catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 102.48(2005):17378-17383.