Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L | |
Gowher, H; Liebert, K; Hermann, A; Xu, GL; Jeltsch, A | |
刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
2005 | |
卷号 | 280期号:14页码:13341-13348 |
通讯作者 | Jeltsch, A (reprint author), Int Jacobs Univ Bremen, Sch Sci & Engn, Campus Ring 1, D-28759 Bremen, Germany.,a.jeltsch@iu-bremen.de |
英文摘要 | Dnmt3L has been identified as a stimulator of the catalytic activity of de novo DNA methyltransferases. It is essential in the development of germ cells in mammals. We show here that Dnmt3L stimulates the catalytic activity of the Dnmt3A and Dnmt3B enzymes by directly binding to their respective catalytic domains via its own C-terminal domain. The catalytic activity of Dnmt3A and -3B was stimulated similar to 15-fold, and Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine (AdoMet). Complex formation between Dnmt3A and Dnmt3L accelerates DNA binding by Dnmt3A 20-fold and lowers its Km for DNA. Interaction of Dnmt3L with Dnmt3A increases the binding of the coenzyme AdoMet to Dnmt3A, and it lowers the Km of Dnmt3A for AdoMet. On the basis of our data we propose a model in which the interaction of Dnmt3A with Dnmt3L induces a conformational change of Dnmt3A that opens the active site of the enzyme and promotes binding of DNA and the AdoMet. We demonstrate that the interaction of Dnmt3A and Dnmt3L is transient, and after DNA binding to Dnmt3A, Dnmt3L dissociates from the complex. Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding to de novo DNA methyltransferases. |
学科主题 | Biochemistry & Molecular Biology |
类目[WOS] | Biochemistry & Molecular Biology |
关键词[WOS] | DNA CYTOSINE-5 METHYLTRANSFERASES ; DE-NOVO METHYLATION ; TRANSCRIPTIONAL REPRESSOR ; IMMUNODEFICIENCY SYNDROME ; MAMMALIAN DEVELOPMENT ; ENZYMATIC-PROPERTIES ; HISTONE DEACETYLASE ; IN-VIVO ; BINDING ; GENE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000228095500020 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/1974] |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Gowher, H,Liebert, K,Hermann, A,et al. Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(14):13341-13348. |
APA | Gowher, H,Liebert, K,Hermann, A,Xu, GL,&Jeltsch, A.(2005).Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L.JOURNAL OF BIOLOGICAL CHEMISTRY,280(14),13341-13348. |
MLA | Gowher, H,et al."Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L".JOURNAL OF BIOLOGICAL CHEMISTRY 280.14(2005):13341-13348. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论