Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes

	Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes
	Guo, LX; Xie, H
刊名	WORLD JOURNAL OF GASTROENTEROLOGY
	2005
卷号	11 期号:9 页码:1345-1350
关键词	p38 Apoptosis Hepatocytes
通讯作者	Xie, H (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Biotherapy, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,hxie@sibs.ac.cn
英文摘要	AIM: To investigate the differential phosphorylation and activation of p38 in hepatocytes by pro-apoptotic Transforming Growth Factor-beta 1 (TGF-beta 1), pro-survival factors Epidermal Growth Factor (EGF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) and the potential mechanisms. METHODS: The phosphorylation and activation of p38 were determined by immunoblotting. Apoptosis was analyzed by morphological staining and observation, FACS analysis of sub-G1 content and DNA fragmentation assay. To quantitatively determine caspase activation, caspase activity assay was performed in vitro. RESULTS: TGF-beta 1-induced apoptosis was associated with the phosphorylation of p38, and SB202190, a specific inhibitor of p38, which was able to inhibit TGF-beta 1-induced caspase activation and apoptosis. TPA and EGF also blocked apoptosis induced by TGF-beta 1. Both of them induced the phosphorylation of p38. The results showed SB202190 had no effect on TGF-beta 1-induced phosphorylation of p38, but effectively inhibited both EGF and TPA-induced phosphorylation of p38. CONCLUSION: Pro-apoptotic TGF-beta 1, anti-apoptotic TPA and EGF induce the phosphorylation of p38 through different mechanisms that can be distinguished by SB202190. The data suggest that TPA and EGF-induced p38 phosphorylation is through an autophosphorylation-dependent mechanism. Since p38 phosphorylation induced by TGF-beta 1 plays an important role in caspase activation and apoptosis, TPA and EGF-induced p38 phosphorylation may not be requisite for their anti-apoptotic function. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.
学科主题	Gastroenterology & Hepatology
类目[WOS]	Gastroenterology & Hepatology
收录类别	SCI
语种	英语
WOS记录号	WOS:000208101400013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1956]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, LX,Xie, H. Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(9):1345-1350.
APA	Guo, LX,&Xie, H.(2005).Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes.WORLD JOURNAL OF GASTROENTEROLOGY,11(9),1345-1350.
MLA	Guo, LX,et al."Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes".WORLD JOURNAL OF GASTROENTEROLOGY 11.9(2005):1345-1350.