A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection

	A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection
	Zhao, MM; An, DR; Huang, GH; He, ZH; Chen, JY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2005
卷号	37 期号:4 页码:248-253
关键词	siRNA HC-Pro tobacco mosaic virus suppressor
通讯作者	An, DR (reprint author), NW Sci & Technol Univ Agr & Forestry, Coll Plant Protect, Shaanxi 712100, Peoples R China.,anmuhong@public.xa.sn.cn ; jychen@sunm.shcnc.ac.cn
英文摘要	Plant viruses encode suppressors of post-transcriptional gene silencing (PTGS), an adaptive defense response that limits virus replication and its spread in plants. The helper component proteinase (HCPro) of the potato virus A (PVA, genus Potyvirus) suppresses PTGS of silenced transgenes. Here, the effect of HC-Pro on siRNA-directed interference in the tobacco mosaic virus (TMV) was examined by using a transient Agrobacterium tumefaciens-based delivery system in intact tissues. It was shown that the interference effect was completely blocked by co-infiltration with HC-Pro plus siRNA constructs in both systemic and hypersensitive hosts. In the system host, all plants agro-infiltrated with HC-Pro plus siRNA constructs displayed the same symptoms as the negative control. Meanwhile, TMV RNA accumulation was found to be abundant in the upper leaves using reverse transcriptase-PCR (RT-PCR) and Northern blot assays. On the contrary, plants agro-infiltrated with the siRNA construct alone were free of symptoms. Therefore, our study suggests that the transient expression of HC-Pro inhibited the siRNA-directed host defenses against TMV infection.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	RNA-MEDIATED INTERFERENCE ; MAMMALIAN-CELLS ; REPLICATION ; PLANTS ; ACCUMULATION ; INHIBITION ; STRATEGY ; DNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000228545300006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1895]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhao, MM,An, DR,Huang, GH,et al. A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(4):248-253.
APA	Zhao, MM,An, DR,Huang, GH,He, ZH,&Chen, JY.(2005).A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(4),248-253.
MLA	Zhao, MM,et al."A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.4(2005):248-253.