High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification

	High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification
	Tu, CJ; Dai, J; Li, SJ; Sheng, QH; Deng, WJ; Xia, QC; Zeng, R
刊名	JOURNAL OF PROTEOME RESEARCH
	2005
卷号	4 期号:4 页码:1265-1273
关键词	plasma immobilized pH gradients isoelectric focusing proteomics linear ion trap mass spectrometry
通讯作者	Zeng, R (reprint author), Chinese Acad Sci, Shangahi Inst Biol Sci, Inst Biochem & Cell Biol, Res Ctr Proteome Anal, 320 YueYang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn
英文摘要	Immobilized pH gradients isoelectric focusing (IPG-IEF) is the first dimension typically used in two-dimensional gel electrophoresis (2-DE). It can also be used on its own in conjunction with tandem mass spectrometry (MS/MS) for the analysis of proteins. Here, we described a strategy combining isoelectric focusing in immobilized pH gradient strips, and mass spectrometry to create a new high-throughput and sensitive detection method. Protein mixture is separated by in-gel IEF, then the entire strip is cut into a set of gel sections. Proteins in each gel section are digested with trypsin, and the resulted pepticles are subjected to reversed-phase high performance liquid chromatography followed by electrospray-linear ion-trap tandem mass analysis. Using this optimized strategy, we have identified 744 distinct human proteins from an IPG strip loaded only 300 mu g of plasma proteins. When compared with other works in published literatures, this study offered a more convenient and sensitive method from gel to mass spectrometry for the separation and identification proteins of complex biological samples.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	PH GRADIENT GELS ; HUMAN SERUM PROTEOME ; 2-DIMENSIONAL ELECTROPHORESIS ; SIGNAL PEPTIDES ; PROTEINS ; CANCER ; PATTERNS ; CHROMATOGRAPHY ; PREDICTION ; SEPARATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000231184200024
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1891]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Tu, CJ,Dai, J,Li, SJ,et al. High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification[J]. JOURNAL OF PROTEOME RESEARCH,2005,4(4):1265-1273.
APA	Tu, CJ.,Dai, J.,Li, SJ.,Sheng, QH.,Deng, WJ.,...&Zeng, R.(2005).High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification.JOURNAL OF PROTEOME RESEARCH,4(4),1265-1273.
MLA	Tu, CJ,et al."High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification".JOURNAL OF PROTEOME RESEARCH 4.4(2005):1265-1273.