Targeting gene-virotherapy of cancer and its prosperity

	Targeting gene-virotherapy of cancer and its prosperity
	Liu, XY
刊名	CELL RESEARCH
	2006
卷号	16 期号:11 页码:879-886
关键词	targeting therapy cancer therapy gene-virotherapy
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	Gene and viral therapies for cancer have shown some therapeutic effects, but there has been a lack of real breakthrough. To achieve the goal of complete elimination of tumor xenograft in animal models, we have developed a new strategy called Targeting Gene-Virotherapy of Cancer, which aims to combine the advantages of both gene therapy and virotherapy. This new strategy has produced stronger anti-tumor effects than either gene or viral therapy alone. A tumor-specific replicative adenovirus vector, designated as ZD55, was constructed by deletion of the 55kDa E1B region of adenovirus. The resulting viral construct not only retains a similar function to ONYX-015 by specifically targeting p53 negative tumors, but also allows for the insertion of various therapeutic genes to form appropriate ZD55 derivatives due to the newly introduced cloning site, a task not feasible with the original ONYX-015 virus. We showed that the anti-tumor effect of one such derivative, ZD55-IL-24, is at least 100 times more potent than that of either ZD55 virotherapy or Ad-IL-24 gene therapy. Nevertheless, complete elimination of tumor mass by the use of ZD55-IL-24 was only observed in some but not all mice, indicating that one therapeutic gene was not sufficient to "cure" these mice. When genes with complementary or synergetic effects were separately cloned into the ZD55 vector and used in combination (designated as the Dual Gene Therapy strategy), much better results were obtained; and it was possible to achieve complete elimination of all the xenograft tumor masses in all mice if two suitable genes were chosen. More comprehensive studies based on this new strategy will likely lead to a protocol for clinical trial. Finally, the concept of Double Controlled Targeting Virus-Dual Gene Therapy for cancer treatment, and the implication of the recent progress in cancer stem cells are also discussed.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	ONCOLYTIC ADENOVIRUS ; ANTITUMOR-ACTIVITY ; PROSPECTIVE IDENTIFICATION ; REPLICATING ADENOVIRUS ; COLORECTAL-CANCER ; SUICIDE GENE ; STEM-CELLS ; BAX GENE ; TUMOR ; PROMOTER
收录类别	SCI
语种	英语
WOS记录号	WOS:000242755000005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1773]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, XY. Targeting gene-virotherapy of cancer and its prosperity[J]. CELL RESEARCH,2006,16(11):879-886.
APA	Liu, XY.(2006).Targeting gene-virotherapy of cancer and its prosperity.CELL RESEARCH,16(11),879-886.
MLA	Liu, XY."Targeting gene-virotherapy of cancer and its prosperity".CELL RESEARCH 16.11(2006):879-886.