A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation

	A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation
	Wang, H; Zheng, HQ; Sha, W; Zeng, R; Xia, QC
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2006
卷号	57 期号:4 页码:827-835
关键词	Arabidopsis thaliana callus cells clinostat rotation proteomics
通讯作者	Zheng, HQ (reprint author), Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, 300 Fenglin Rd, Shanghai 200032, Peoples R China.,hqzheng@sippe.ac.cn
英文摘要	Callus cells of Arabidopsis thaliana (cv. Landsberg erecta) were exposed for 8 h to a horizontal clinostat rotation (H, simulated weightlessness), a vertical clinostat rotation (V, clinostat control), or a stationary control (S) growth condition. The amount of glucose and fructose apparently decreased, while starch content increased in the H compared with the V- and S-treated cells. In order to investigate the influences of clinostat rotation on the cellular proteome further, the proteome alterations induced by horizontal and vertical clinostat rotation have been comparatively analysed by high-resolution two-dimensional (2-D) gel electrophoresis and mass spectrometry. Image analysis of silver-stained 2-D gels revealed that 80 protein spots showed quantitative and qualitative variations that were significantly (P < 0.01) and reproducibly different between the clinorotated (H or V) and the stationary control samples. Protein spots excised from 2-D gels were analysed by microbe high performance liquid chromatography-ion trap-mass spectrometry (LC-IT-MS) to obtain the tandem mass (MS/MS) spectra. 18 protein spots, which showed significant expression alteration only under the H condition compared with those under V and S conditions, were identified. Of these proteins, seven were involved in stress responses, and four protein spots were identified as key enzymes in carbohydrate metabolism and lipid biosynthesis. Two reversibly glycosylated cell wall proteins were down-regulated in the H samples. Other proteins such as protein disulphide isomerase, transcription initiation factor IIF, and two ribosomal proteins also exhibited altered expression under the H condition. The data presented in this study illustrate that clinostat rotation of Arabidopsis callus cells has a significant impact on the expression of proteins involved in general stress responses, metabolic pathways, gene activation/transcription, protein synthesis, and cell wall biosynthesis.
学科主题	Plant Sciences
类目[WOS]	Plant Sciences
关键词[WOS]	ORNITHINE-DELTA-AMINOTRANSFERASE ; PROTEIN DISULFIDE-ISOMERASE ; STIMULATED ROOT CAPS ; SALT-STRESS ; ISOCITRATE DEHYDROGENASE ; PROLINE ACCUMULATION ; GENE-EXPRESSION ; DESATURASE GENE ; ALTERED GRAVITY ; HUMAN HEPATOMA
收录类别	SCI
语种	英语
WOS记录号	WOS:000235771700012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1713]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, H,Zheng, HQ,Sha, W,et al. A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation[J]. JOURNAL OF EXPERIMENTAL BOTANY,2006,57(4):827-835.
APA	Wang, H,Zheng, HQ,Sha, W,Zeng, R,&Xia, QC.(2006).A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation.JOURNAL OF EXPERIMENTAL BOTANY,57(4),827-835.
MLA	Wang, H,et al."A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation".JOURNAL OF EXPERIMENTAL BOTANY 57.4(2006):827-835.