Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling

	Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling
	Yao, G; Chen, WY; Luo, HB; Jiang, QF; Xia, ZX; Zang, L; Zuo, JP; Wei, X; Chen, ZJ; Shen, X
刊名	INTERNATIONAL IMMUNOLOGY
	2006
卷号	18 期号:1 页码:19-29
关键词	11B.11 core functional region of IL-4 IL-4 molecular modeling peptide phage display
通讯作者	Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,xiazx@mail.sloc.ac.cn ; bsun@sibs.ac.cn
英文摘要	Murine IL-4 is a pleiotropic cytokine with undefined core functional region for eliciting downstream signaling. We used molecular modeling to predict the binding sites recognized by an anti-IL-4-neutralizing mAb (11B.11) and peptide phage display to delineate their makeup. The results of these approaches were confirmed by site-directed mutagenesis analysis. The results suggest that the amino acid residues spanning from 79 to 86 (QRLFRAFR) on IL-4 are of the major binding site for 11B.11. Furthermore, the functional experiments demonstrate that the residues R80, R83 and R86, which are located in the helix C of murine IL-4, play a crucial role in binding to the IL-4R alpha-chain. Taken together, a new core functional region of murine IL-4 is identified, which provides new insight into the interaction between IL-4 and IL-4R alpha. In addition, the results demonstrate that 11B.11 binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	AMINO-ACID-RESIDUES ; MONOCLONAL-ANTIBODY ; HUMAN INTERLEUKIN-4 ; SIGNAL-TRANSDUCTION ; IN-VIVO ; MOUSE INTERLEUKIN-4 ; CRYSTAL-STRUCTURE ; RECEPTOR-BINDING ; SWISS-MODEL ; GAMMA-CHAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000234436500003
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1655]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yao, G,Chen, WY,Luo, HB,et al. Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling[J]. INTERNATIONAL IMMUNOLOGY,2006,18(1):19-29.
APA	Yao, G.,Chen, WY.,Luo, HB.,Jiang, QF.,Xia, ZX.,...&Sun, B.(2006).Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling.INTERNATIONAL IMMUNOLOGY,18(1),19-29.
MLA	Yao, G,et al."Identification of core functional region of murine IL-4 using peptide phage display and molecular modeling".INTERNATIONAL IMMUNOLOGY 18.1(2006):19-29.