Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells

	Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells
	Jiang, H; Hou, CH; Zhang, SB; Xie, HY; Zhou, WY; Jin, QH; Cheng, XD; Qian, RL; Zhang, XJ
刊名	EUROPEAN JOURNAL OF PHARMACOLOGY
	2007
卷号	559 期号:1 页码:98-108
关键词	matrine apoptosis K562 E2F-1 mitochondria
通讯作者	Zhang, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, 320 YueYang Rd, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn
英文摘要	Matrine is a major component of Sophora Flavescens and has been reported to stimulate differentiation of erythroleukemia cells. Here we show that matrine inhibits cell proliferation or induces apoptosis in a cell type-specific manner. The latter effect was investigated in more detail in the p53 deficient erythroleukemia cell line, K562. Martine exposure induced apoptosis in a time- and dose-dependent manner in these cells. Interestingly, co-treatment with etoposide potentiated apoptosis. Further analysis of matrine-induced apoptotic changes revealed that E2F-1 and Apaf-1 were upregulated, whereas Rb was downregulated after 24 h of exposure. This was followed by Bax translocation, cytochrome c release, and caspase-9 and -3 activation. These results demonstrate that matrine triggers apoptosis of K562 cells primarily through the mitochondrial pathway and that matrine is a potential anti-tumor drug. (c) 2007 Elsevier B.V. All rights reserved.
学科主题	Pharmacology & Pharmacy
类目[WOS]	Pharmacology & Pharmacy
关键词[WOS]	E2F-1-INDUCED APOPTOSIS ; LEUKEMIA-CELLS ; P53 ; DEATH ; BAX ; ROLES ; GENE ; RB ; OVEREXPRESSION ; ACTIVATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000244969800002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1637]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Jiang, H,Hou, CH,Zhang, SB,et al. Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2007,559(1):98-108.
APA	Jiang, H.,Hou, CH.,Zhang, SB.,Xie, HY.,Zhou, WY.,...&Zhang, XJ.(2007).Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells.EUROPEAN JOURNAL OF PHARMACOLOGY,559(1),98-108.
MLA	Jiang, H,et al."Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells".EUROPEAN JOURNAL OF PHARMACOLOGY 559.1(2007):98-108.