Regulation and pharmacokinetics of inducible recombinant TRAIL expression

	Regulation and pharmacokinetics of inducible recombinant TRAIL expression
	Dong, AW; Hu, J; Zhao, LL; Xu, HL; Liu, XY
刊名	CANCER BIOLOGY & THERAPY
	2007
卷号	6 期号:12 页码:1978-1985
关键词	recombinant TRAIL RU 486 regulatory system expression kinetics cancer gene therapy
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn
英文摘要	TRAIL is a potent antitumor agent, but its potential toxicity to normal human tissues limits its clinical applications in future. Therapy of human tumors might benefit from the use of vectors enabling tight control of TRAIL expression in vivo. To this aim, we constructed an adenoviral vector carrying the RU486-dependent gene switch system for the regulable expression of recombinant TRAIL. Only was apoptotic recombinant TRAIL expressed and cytotoxicity observed upon binding of RU 486 to the inducible promoter. Expression levels and kinetics of recombinant TRAIL expression could be achieved by modulating the concentration of the inducer. As a broad implication, our data provide an alternative approach to circumvent the potential toxicity of TRAIL in future human trials and this system may be utilized to treat human cancer using a long-term expression vector.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	PRIMARY HUMAN HEPATOCYTES ; GENE-TRANSFER ; TRIMERIC TRAIL ; TUMOR-GROWTH ; IN-VIVO ; APOPTOSIS ; LIGAND ; THERAPY ; CELLS ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000253944900029
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1620]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Dong, AW,Hu, J,Zhao, LL,et al. Regulation and pharmacokinetics of inducible recombinant TRAIL expression[J]. CANCER BIOLOGY & THERAPY,2007,6(12):1978-1985.
APA	Dong, AW,Hu, J,Zhao, LL,Xu, HL,&Liu, XY.(2007).Regulation and pharmacokinetics of inducible recombinant TRAIL expression.CANCER BIOLOGY & THERAPY,6(12),1978-1985.
MLA	Dong, AW,et al."Regulation and pharmacokinetics of inducible recombinant TRAIL expression".CANCER BIOLOGY & THERAPY 6.12(2007):1978-1985.