rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells

	rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells
	Yuan, YY; Gao, X; Guo, N; Zhang, H; Xie, ZQ; Jin, ML; Li, BM; Yu, L; Jing, NH
刊名	CELL RESEARCH
	2007
卷号	17 期号:11 页码:919-932
关键词	PIPPase intracellular membrane trafficking neurite outgrowth
通讯作者	Jin, ML (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Biotechnol Res Ctr, 500 Cao Bao Rd, Shanghai 200233, Peoples R China.,njing@sibs.ac.cn
英文摘要	Sac domain-containing proteins belong to a newly identified family of phosphoinositide phosphatases (the PIPPase family). Despite well-characterized enzymatic activity, the biological functions of this mammalian Sac domain PIPPase family remain largely unknown. We identified a novel Sac domain-containing protein, rat Sac3 (rSac3), which is widely expressed in various tissues and localized to the endoplasmic reticulum, Golgi complex and recycling endosomes. rSac3 displays PIPPase activity with PI(3)P, PI(4)P and PI(3,5)P-2 as substrates in vitro, and a mutation in the catalytic core of the Sac domain abolishes its enzymatic activity. The expression of rSac3 is upregulated during nerve growth factor (NGF)-stimulated PC 12 cell neuronal differentiation, and overexpression of this protein promotes neurite outgrowth in PC 12 cells. Conversely, inhibition of rSac3 expression by antisense oligonucleotides reduces neurite outgrowth of NGF-stimulated PC 12 cells, and the active site mutation of rSac3 eliminates its neurite-outgrowth-promoting activity. These results indicate that rSac3 promotes neurite outgrowth in differentiating neurons through its PIPPase activity, suggesting that Sac domain PIPPase proteins may participate in forward membrane trafficking from the endoplasmic reticulum and Golgi complex to the plasma membrane, and may function as regulators of this crucial process of neuronal cell growth and differentiation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	SYNAPTIC VESICLE ENDOCYTOSIS ; CLATHRIN-MEDIATED ENDOCYTOSIS ; MICROSOMAL ATP TRANSPORT ; MULTIVESICULAR BODY ; LIPID PHOSPHATASE ; NERVE-TERMINALS ; SYNAPTOJANIN 2 ; BINDING ; PROTEIN ; GOLGI
收录类别	SCI
语种	英语
WOS记录号	WOS:000251953300005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1608]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yuan, YY,Gao, X,Guo, N,et al. rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells[J]. CELL RESEARCH,2007,17(11):919-932.
APA	Yuan, YY.,Gao, X.,Guo, N.,Zhang, H.,Xie, ZQ.,...&Jing, NH.(2007).rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells.CELL RESEARCH,17(11),919-932.
MLA	Yuan, YY,et al."rSac3, a novel Sac domain phosphoinositide phosphatase, promotes neurite outgrowth in PC12 cells".CELL RESEARCH 17.11(2007):919-932.