Characterization and expression of mouse Cdc50c during spermatogenesis

	Characterization and expression of mouse Cdc50c during spermatogenesis
	Xu, P; Ding, XY
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2007
卷号	39 期号:10 页码:739-744
关键词	spermatogenesis infertility testis cell polarity Cdc50c
通讯作者	Ding, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Stem Cell Biol,Lab Mol & Cell Biol, Beijing 100864, Peoples R China.,xyding@sunm.shcnc.ac.cn
英文摘要	Cdc50p is a transmembrane protein required for polarized growth in Saccharomyces cerevisiae. The knowledge on physiological functions of its mammalian homologs, however, is limited. Using database analysis, we identified one mouse testis expressed sequence tag, named Cdc50c, encoding a previously uncharacterized homolog of Cdc50p. Similar to yeast Cdc50p, the putative Cdc50c protein contains three transmembrane spanning regions. Its orthologs are present in many species such as fish, avian and human, suggesting its evolutionary conservation. In multitissue reverse transcription-polymerase chain reaction analyses the mRNA for Cdc50c was predominately detected in testis. The onset of the gene expression coincides with the first appearance of spermatocytes during testicular development. In situ hybridization analyses revealed that Cdc50c mRNA localized in pachytene spermatocytes and round and elongated spermatids. Our data suggest that Cdc50c might play important roles during spermatogenesis.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	PHOSPHOLIPID TRANSLOCATION ; SACCHAROMYCES-CEREVISIAE ; ACTIN CYTOSKELETON ; POLARIZED GROWTH ; PROTEIN ; YEAST ; DIFFERENTIATION ; SPERMATIDS ; FAMILY ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000250360500002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1600]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, P,Ding, XY. Characterization and expression of mouse Cdc50c during spermatogenesis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(10):739-744.
APA	Xu, P,&Ding, XY.(2007).Characterization and expression of mouse Cdc50c during spermatogenesis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(10),739-744.
MLA	Xu, P,et al."Characterization and expression of mouse Cdc50c during spermatogenesis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.10(2007):739-744.