P-selectin primes leukocyte integrin activation during inflammation

	P-selectin primes leukocyte integrin activation during inflammation
	Wang, HB; Wang, JT; Zhang, L; Geng, ZH; Xu, WL; Xu, T; Huo, YQ; Zhu, XL; Plow, EF; Chen, M
刊名	NATURE IMMUNOLOGY
	2007
卷号	8 期号:8 页码:882-892
通讯作者	Geng, JG (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China.,chenm@sibs.ac.cn ; genglab@gmail.com
英文摘要	Selectins mediate leukocyte rolling and prime leukocytes for integrin-mediated leukocyte adhesion. However, neither the in vivo importance of nor the signaling pathway by which selectin-mediated integrin activation occurs has been determined. We report here that P-selectin-deficient mice manifested impaired leukocyte adhesion, which was 'rescued' by soluble P-selectin. Mechanistically, the cytoplasmic domain of P-selectin glycoprotein ligand 1 formed a constitutive complex with Nef-associated factor 1. After binding of P-selectin, Src kinases phosphorylated Nef-associated factor 1, which recruited the phosphoinositide-3-OH kinase p85-p110 delta heterodimer and resulted in activation of leukocyte integrins. Inhibition of this signal-transduction pathway diminished the adhesion of leukocytes to capillary venules and suppressed peritoneal infiltration of leukocytes. Our data demonstrate the functional importance of this newly identified signaling pathway mediated by P-selectin glycoprotein ligand 1.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	ALPHA-INDUCED INFLAMMATION ; GLYCOPROTEIN LIGAND 1 ; FIRM ADHESION ; MUTANT MICE ; SRC KINASES ; NEUTROPHILS ; CELL ; BINDING ; EXPRESSION ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000248169400016
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1590]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, HB,Wang, JT,Zhang, L,et al. P-selectin primes leukocyte integrin activation during inflammation[J]. NATURE IMMUNOLOGY,2007,8(8):882-892.
APA	Wang, HB.,Wang, JT.,Zhang, L.,Geng, ZH.,Xu, WL.,...&Geng, JG.(2007).P-selectin primes leukocyte integrin activation during inflammation.NATURE IMMUNOLOGY,8(8),882-892.
MLA	Wang, HB,et al."P-selectin primes leukocyte integrin activation during inflammation".NATURE IMMUNOLOGY 8.8(2007):882-892.