Calcineurin mediates acetylcholinesterase expression during calcium ionophore A23187-induced HeLa cell apoptosis

	Calcineurin mediates acetylcholinesterase expression during calcium ionophore A23187-induced HeLa cell apoptosis
	Zhu, H; Gao, W; Jiang, H; Wu, J; Shi, YF; Zhang, XJ
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
	2007
卷号	1773 期号:4 页码:593-602
关键词	acetylcholinesterase apoptosis calpain calcineurin NFAT
通讯作者	Zhang, XJ (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, 320 YueYang Rd, Shanghai 200031, Peoples R China.,xjzhang@sibs.ac.cn
英文摘要	We previously reported that acetylcholinesterase plays a critical role in apoptosis and its expression is regulated by Ca(2+) mobilization. In the present study, we show that activated calpain, a cytosolic calcium-activated cysteine protease, and calcineurin, a calcium-dependent protein phosphatase, regulate acetylcholinesterase expression during A23187-induced apoptosis. The calpain inhibitor, calpeptin, and the calcineurin inhibitors, FK506 and cyclosporine A, inhibited acetylcholinesterase expression at both mRNA and protein levels and suppressed the activity of the human acetylcholinesterase promoter. In contrast, overexpression of constitutively active calcineurin significantly activated the acetylcholinesterase promoter. Furthermore, we identify a role for the transcription factor NFAT (nuclear factor of activated T cells), a calcineurin target, in regulating the acetylcholinesterase promoter during ionophore-induced apoptosis. Overexpression of human NFATc3 and NFATc4 greatly increased the acetylcholinesterase promoter activity in HeLa cells treated with A23187. Overexpression of constitutive nuclear NFATc4 activated the acetylcholinesterase promoter independent of A23187, whereas overexpression of dominant-negative NFAT blocked A23187-induced acetylcholinesterase promoter activation. These results indicate that calcineurin mediates acetylcholinesterase expression during apoptosis. (c) 2007 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	MESSENGER-RNA STABILITY ; ALZHEIMERS-DISEASE ; NEUROMUSCULAR-JUNCTIONS ; NEURONAL CELLS ; CALPAIN-I ; ACTIVATION ; BRAIN ; CLEAVAGE ; PROTEIN ; NFAT
收录类别	SCI
语种	英语
WOS记录号	WOS:000245784700014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1552]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhu, H,Gao, W,Jiang, H,et al. Calcineurin mediates acetylcholinesterase expression during calcium ionophore A23187-induced HeLa cell apoptosis[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2007,1773(4):593-602.
APA	Zhu, H,Gao, W,Jiang, H,Wu, J,Shi, YF,&Zhang, XJ.(2007).Calcineurin mediates acetylcholinesterase expression during calcium ionophore A23187-induced HeLa cell apoptosis.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1773(4),593-602.
MLA	Zhu, H,et al."Calcineurin mediates acetylcholinesterase expression during calcium ionophore A23187-induced HeLa cell apoptosis".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1773.4(2007):593-602.