Differential expression of neutrophilic granule proteins between Th1 and Th2 cells

	Differential expression of neutrophilic granule proteins between Th1 and Th2 cells
	Xie, D; Liu, ZD; Li, ZH; Ji, YY; Chen, JW; Sun, B
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2007
卷号	39 期号:1 页码:67-72
关键词	neutrophilic granule protein cystatin Th2 cell
通讯作者	Sun, B (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn
英文摘要	T helper cell type 1 (Th1) and 2 (Th2) play central roles in immune regulation. To identify the novel genes differentially expressed between Th1 and Th2 cells, CD4+ T cells were isolated from DO11.10 transgenic mice and induced under Th1 or Th2 conditions. Microarray showed differential expression of neutrophilic granule proteins (NGP) between Th1 and Th2 cells. NGP was first identified as a myeloid-specific granule protein with homology to the cystatin superfamily. Here we confirmed greater expression of NGP in Th2 cells by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction analysis. We also showed that the expression of NGP mRNA had a peak expression after 5 d culture under Th2- but not Th1-biasing conditions. Antibody against NGP was prepared, and in concert with the results of mRNA analysis, the level of NGP protein in Th2 cells detected by Western blot analysis was also higher than that in Th1 cells. GFP-NGP fusion proteins overexpressed in HeLa cells were localized to the cytoplasm. These results suggest NGP is a novel marker distinguishing Th2 from Th1 cells and maybe a novel cytokine secreted by Th2 cells.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CYSTEINE PROTEASE INHIBITOR ; IMMUNE-RESPONSES ; CYSTATIN-SA ; PROLIFERATION ; MODULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000243881000009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1510]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xie, D,Liu, ZD,Li, ZH,et al. Differential expression of neutrophilic granule proteins between Th1 and Th2 cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(1):67-72.
APA	Xie, D,Liu, ZD,Li, ZH,Ji, YY,Chen, JW,&Sun, B.(2007).Differential expression of neutrophilic granule proteins between Th1 and Th2 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(1),67-72.
MLA	Xie, D,et al."Differential expression of neutrophilic granule proteins between Th1 and Th2 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.1(2007):67-72.