Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation

	Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation
	Pan, WJ; Choi, SC; Wang, H; Qin, YB; Volpicelli-Daley, L; Swan, L; Lucast, L; Khoo, C; Zhang, XW; Li, L
刊名	SCIENCE
	2008
卷号	321 期号:5894 页码:1350-1353
通讯作者	Wu, DQ (reprint author), Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA.,dan.wu@yale.edu
英文摘要	The canonical Wnt-beta-catenin signaling pathway is initiated by inducing phosphorylation of one of the Wnt receptors, low- density lipoprotein receptor- related protein 6 ( LRP6), at threonine residue 1479 ( Thr(1479)) and serine residue 1490 (Ser(1490)). By screening a human kinase small interfering RNA library, we identified phosphatidylinositol 4- kinase type II alpha and phosphatidylinositol- 4- phosphate 5- kinase type I (PIP5KI) as required for Wnt3a-induced LRP6 phosphorylation at Ser1490 in mammalian cells and confirmed that these kinases are important for Wnt signaling in Xenopus embryos. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates [PtdIns (4,5)P(2)] through frizzled and dishevelled, the latter of which directly interacted with and activated PIP5KI. In turn, PtdIns (4,5)P(2) regulated phosphorylation of LRP6 at Thr(1479) and Ser(1490). Therefore, our study reveals a signaling mechanism for Wnt to regulate LRP6 phosphorylation.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	WNT SIGNALING PATHWAY ; PLASMA-MEMBRANE ; DISEASE ; DOMAIN ; ACTIVATION ; CELLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000258914300049
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1484]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Pan, WJ,Choi, SC,Wang, H,et al. Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation[J]. SCIENCE,2008,321(5894):1350-1353.
APA	Pan, WJ.,Choi, SC.,Wang, H.,Qin, YB.,Volpicelli-Daley, L.,...&Wu, DQ.(2008).Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation.SCIENCE,321(5894),1350-1353.
MLA	Pan, WJ,et al."Wnt3a-mediated formation of phosphatidylinositol 4,5-bisphosphate regulates LRP6 phosphorylation".SCIENCE 321.5894(2008):1350-1353.