Efg1-mediated Recruitment of NuA4 to Promoters Is Required for Hypha-specific Swi/Snf Binding and Activation in Candida albicans

	Efg1-mediated Recruitment of NuA4 to Promoters Is Required for Hypha-specific Swi/Snf Binding and Activation in Candida albicans
	Lu, Y; Su, C; Mao, XM; PalaRaniga, P; Liu, HP; Chen, JY
刊名	MOLECULAR BIOLOGY OF THE CELL
	2008
卷号	19 期号:10 页码:4260-4272
通讯作者	Liu, HP (reprint author), Univ Calif Irvine, Coll Med, Dept Biol Chem, Irvine, CA 92697 USA.,h4liu@uci.edu ; jychen@sunm.shcnc.ac.cn
英文摘要	Efg1 is essential for hyphal development and virulence in the human pathogenic fungus Candida albicans. How Efg1 regulates gene expression is unknown. Here, we show that Efg1 interacts with components of the nucleosome acetyltransferase of H4 (NuA4) histone acetyltransferase (HAT) complex in both yeast and hyphal cells. Deleting YNG2, a subunit of the NuA4 HAT module, results in a significant decrease in the acetylation level of nucleosomal H4 and a profound defect in hyphal development, as well as a defect in the expression of hypha-specific genes. Using chromatin immunoprecipitation, Efg1 and the NuA4 complex are found at the UAS regions of hypha-specific genes in both yeast and hyphal cells, and Efg1 is required for the recruitment of NuA4. Nucleosomal H4 acetylation at the promoters peaks during initial hyphal induction in an Efg1-dependent manner. We also find that Efg1 bound to the promoters of hypha-specific genes is critical for recruitment of the Swi/Snf chromatin remodeling complex during hyphal induction. Our data show that the recruitment of the NuA4 complex by Efg1 to the promoters of hypha-specific genes is required for nucleosomal H4 acetylation at the promoters during hyphal induction and for subsequent binding of Swi/Snf and transcriptional activation.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	HISTONE ACETYLTRANSFERASE COMPLEX ; CHROMATIN REMODELING COMPLEX ; EFG1 REGULATOR PROTEIN ; CELL-CYCLE ; FILAMENTOUS GROWTH ; TRANSCRIPTIONAL ACTIVATION ; ORDERED RECRUITMENT ; GENE-EXPRESSION ; FUNGAL PATHOGEN ; IN-VIVO
收录类别	SCI
语种	英语
WOS记录号	WOS:000260472200022
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1445]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lu, Y,Su, C,Mao, XM,et al. Efg1-mediated Recruitment of NuA4 to Promoters Is Required for Hypha-specific Swi/Snf Binding and Activation in Candida albicans[J]. MOLECULAR BIOLOGY OF THE CELL,2008,19(10):4260-4272.
APA	Lu, Y,Su, C,Mao, XM,PalaRaniga, P,Liu, HP,&Chen, JY.(2008).Efg1-mediated Recruitment of NuA4 to Promoters Is Required for Hypha-specific Swi/Snf Binding and Activation in Candida albicans.MOLECULAR BIOLOGY OF THE CELL,19(10),4260-4272.
MLA	Lu, Y,et al."Efg1-mediated Recruitment of NuA4 to Promoters Is Required for Hypha-specific Swi/Snf Binding and Activation in Candida albicans".MOLECULAR BIOLOGY OF THE CELL 19.10(2008):4260-4272.