Tetrandrine protects mice from concanavalin A-induced hepatitis through inhibiting NF-kappa B activation

	Tetrandrine protects mice from concanavalin A-induced hepatitis through inhibiting NF-kappa B activation
	Feng, DC; Mei, YH; Wang, Y; Zhang, BH; Wang, C; Xu, LY
刊名	IMMUNOLOGY LETTERS
	2008
卷号	121 期号:2 页码:127-133
关键词	Tetrandrine Concanavalin A-induced hepatitis NF-kappa B
通讯作者	Xu, LY (reprint author), Bldg 1 Room 308,225 S Chongqing Rd, Shanghai 200025, Peoples R China.,lyxu@sibs.ac.cn
英文摘要	Tetrandrine (TET) is the major pharmacologically active compound of Chinese herb Stephania tetrandra S Moore, which has been used traditionally for the treatment of rheumatic disorders, silicosis and hypertension, Concanavalin A (ConA)-induced hepatitis (CIH) is a T-cell-dependent hepatitis and a well-established animal model for studying the mechanisms and therapy of immune-mediated hepatotoxicity. The aim of this study was to investigate whether TET could protect mice from CIH. C57BL/6 mice were injected with ConA to induce CIH pretreated with or without TET. Liver injury was assessed biochemically and histologically. Levels of plasma cytokines and the expressions of chemokine messenger RNA (mRNA) in the liver were determined. We found that pretreatment of mice with TET markedly reduced plasma transaminase release and the severity of liver damage. We further investigated the mechanisms of the protective effects of TET. When CIH-induced mice pretreated with TET, the increases of plasma concentrations of TNF-alpha, IFN-gamma, IL-12 and IL-4 were dramatically attenuated: at the same time, IFN-inducible protein-10 and macrophage inflammatory protein-1 a expressions in liver were decreased. Furthermore, TET inhibited NF-kappa B activity, the critical transcriptional factor of the above mentioned inflammatory cytokines, by preventing the activation Of I kappa B alpha kinasea. (IKK alpha) and then inhibiting phosphorylation Of I kappa B alpha to stabilize I kappa B alpha in intrahepatic leukocytes. In conclusion, TET is able to prevent T-cell-mediated liver injury in vivo. The beneficial effect may depend on suppressing the production of various inflammatory mediators in the liver through inhibiting of NF-kappa B activation. (C) 2008 Elsevier B.V. All rights reserved.
学科主题	Immunology
类目[WOS]	Immunology
关键词[WOS]	TUMOR-NECROSIS-FACTOR ; PLANT ALKALOID TETRANDRINE ; CELL-MEDIATED HEPATITIS ; T-CELL ; INTERFERON-GAMMA ; LIVER-INJURY ; BISBENZYLISOQUINOLINE ALKALOIDS ; INDUCIBLE PROTEIN-10 ; SIGNALING PATHWAY ; TNF-ALPHA
收录类别	SCI
语种	英语
WOS记录号	WOS:000262769300008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1328]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Feng, DC,Mei, YH,Wang, Y,et al. Tetrandrine protects mice from concanavalin A-induced hepatitis through inhibiting NF-kappa B activation[J]. IMMUNOLOGY LETTERS,2008,121(2):127-133.
APA	Feng, DC,Mei, YH,Wang, Y,Zhang, BH,Wang, C,&Xu, LY.(2008).Tetrandrine protects mice from concanavalin A-induced hepatitis through inhibiting NF-kappa B activation.IMMUNOLOGY LETTERS,121(2),127-133.
MLA	Feng, DC,et al."Tetrandrine protects mice from concanavalin A-induced hepatitis through inhibiting NF-kappa B activation".IMMUNOLOGY LETTERS 121.2(2008):127-133.