A Unique Insertion in the CP1 Domain of Giardia lamblia Leucyl-tRNA Synthetase

	A Unique Insertion in the CP1 Domain of Giardia lamblia Leucyl-tRNA Synthetase
	Zhou, XL; Yao, P; Ruan, LL; Zhu, B; Luo, J; Qu, LH; Wang, ED
刊名	BIOCHEMISTRY
	2009
卷号	48 期号:6 页码:1340-1347
通讯作者	Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Grad Sch,State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn
英文摘要	Leucyl-tRNA synthetase (LeuRS) catalyzes the esterification of the tRNA(Leu) isoacceptor with leucine. It contains a large insertion domain, connective peptide 1 (CP1), for amino acid editing. Here, we cloned the gene encoding LeuRS from Giardia lamblia (GlLeuRS), one of the most ancient eukaryotes. GlLeuRS was purified from an Escherichia coli overproduction strain, and its properties were investigated. The isolated CP1 domain of GlLeuRS (GlLeuRS-CP1) was an active protein for editing mischarged G. lamblia tRNA(Leu)(AAG) (GltRNA(Leu)). Insertion of 49 amino acid residues within the CP1 domain (the so-called 49-amino acid motif) was important for the optimal aminoacylation activity of GlLeuRS and was crucial for the editing capacity of GlLeuRS-CP1. Additionally, the motif can confer editing activity on the editing-defective isolated CP1 domain from E. coli LeuRS (EcLeuRS-CP1). We also found that GlLeuRS could not rescue a Saccharomyces cerevisiae leuS null strain, suggesting different recognition modes for these two LeuRSs with respect to tRNA(Leu).
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	AQUIFEX-AEOLICUS ; CRYSTAL-STRUCTURE ; TRNA(LEU) ; AMINOACYLATION ; SELECTION ; COMPLEX ; MOTIFS
收录类别	SCI
语种	英语
WOS记录号	WOS:000263226400021
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1214]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, XL,Yao, P,Ruan, LL,et al. A Unique Insertion in the CP1 Domain of Giardia lamblia Leucyl-tRNA Synthetase[J]. BIOCHEMISTRY,2009,48(6):1340-1347.
APA	Zhou, XL.,Yao, P.,Ruan, LL.,Zhu, B.,Luo, J.,...&Wang, ED.(2009).A Unique Insertion in the CP1 Domain of Giardia lamblia Leucyl-tRNA Synthetase.BIOCHEMISTRY,48(6),1340-1347.
MLA	Zhou, XL,et al."A Unique Insertion in the CP1 Domain of Giardia lamblia Leucyl-tRNA Synthetase".BIOCHEMISTRY 48.6(2009):1340-1347.