Biosynthesis and characterization of typical fibroin crystalline polypeptides of silkworm Bombyx mori

	Biosynthesis and characterization of typical fibroin crystalline polypeptides of silkworm Bombyx mori
	Wang, JN; Yan, SQ; Lu, CD; Bai, L
刊名	MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
	2009
卷号	29 期号:4 页码:1321-1325
关键词	Fibroin crystalline Silkworm Genes Fusion protein Thrombin ThT
通讯作者	Wang, JN (reprint author), Soochow Univ, Coll Mat Engn, Suzhou 215021, Peoples R China.,wangjn@suda.edu.cn
英文摘要	We aimed to investigate the self-organization/self-assembly mechanisms of silkworm fibroin-based material. In the present study, for the first time, we designed and multimerized four DNA "monomer" sequences from structurally simple fibroin crystalline peptides or analog, [GAGAGX] (X = A, S, Y and V) to encode polypeptides [GAGAGX](16) (eGA, eGS, eGY and eGV) using a "head-to-tail" construction strategy. Multimers were cloned into pGEX-KG and fusion proteins GST-[GAGAGX](16) (KGA, KGS, KGY and KGV) were efficiently expressed in Escherichia coli. These fusion proteins were isolated and purified by GST affinity chromatography and confirmed by SDS-PAGE and Western blot analysis using antibody reactive to GST The polypeptides were cleavaged from GST fusion proteins by digesting with thrombin enzyme. The composition of the four polypeptides was confirmed by composition analysis of amino acids, and their abilities to form beta-sheet structure were determined by ThT fluorescence Spectral analysis. The content of beta-sheet among the four polypeptides followed the order: eGS>eGV>eGY>eGA. (C) 2008 Elsevier B.V. All rights reserved.
学科主题	Materials Science
类目[WOS]	Materials Science, Multidisciplinary
关键词[WOS]	CHAINS ; DEGRADATION ; SCAFFOLDS ; PEPTIDES ; PROTEIN ; SHEETS ; HEAVY ; ACID
收录类别	SCI
语种	英语
WOS记录号	WOS:000269139900043
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1175]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, JN,Yan, SQ,Lu, CD,et al. Biosynthesis and characterization of typical fibroin crystalline polypeptides of silkworm Bombyx mori[J]. MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS,2009,29(4):1321-1325.
APA	Wang, JN,Yan, SQ,Lu, CD,&Bai, L.(2009).Biosynthesis and characterization of typical fibroin crystalline polypeptides of silkworm Bombyx mori.MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS,29(4),1321-1325.
MLA	Wang, JN,et al."Biosynthesis and characterization of typical fibroin crystalline polypeptides of silkworm Bombyx mori".MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 29.4(2009):1321-1325.