Suppression of cancer growth in mice by adeno-associated virus vector-mediated IFN-beta expression driven by hTERT promoter

	Suppression of cancer growth in mice by adeno-associated virus vector-mediated IFN-beta expression driven by hTERT promoter
	He, LF; Wang, YG; Xiao, T; Zhang, KJ; Li, GC; Gu, JF; Chu, L; Tang, WH; Tan, WS; Liu, XY
刊名	CANCER LETTERS
	2009
卷号	286 期号:2 页码:196-205
关键词	IFN-beta Adeno-associated virus hTERT promoter Antitumor efficacy
通讯作者	Liu, XY (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Lab Canc Therapy, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wstan@ecust.edu.cn ; xyliu@sibs.ac.cn
英文摘要	Adeno-associated virus (AAV) has rapidly become a promising gene delivery vehicle for its excellent advantages of non-immunogenic, low pathogenicity and long-term gene expression in vivo. However, a major obstacle in development of effective AAV vector is the lack of tissue specificity, which caused low efficiency of AAV transfer to target cells. The application of human telomerase reverse transcriptase (hTERT) promoter is a prior targeting strategy for AAV in cancer gene therapy as hTERT activity is transcriptionally upregulated in most cancer cells. In the present work, we investigated whether AAV-mediated human interferon beta (IFN-beta) gene driven by hTERT promoter could specifically express in tumor cells and suppress tumor cell growth. Our data demonstrated that hTERT promoter-driven IFN-beta expression was the tumor-specific, decreased the cell viability of tumor cells but not normal cells, and induced tumor cell apoptosis via activation of caspase pathway and release of cytochrome c. AAV-mediated IFN-beta expression driven by hTERT promoter significantly suppressed the growth of colorectal cancer and lung cancer xenograft in mice and resulted in tumor cells death in vivo. These data suggested that AAVs in combination with hTERT-mediated IFN-beta expression could exert potential antitumor activity and provide a novel targeting approach to clinical gene therapy of varieties of cancers. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
学科主题	Oncology
类目[WOS]	Oncology
关键词[WOS]	HUMAN HEPATOCELLULAR-CARCINOMA ; CELLS IN-VITRO ; TELOMERASE ACTIVITY ; GENE-THERAPY ; INTERFERON-BETA ; ADENOVIRAL VECTOR ; SYSTEMIC DELIVERY ; VIRAL VECTOR ; NEUROBLASTOMA ; APOPTOSIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000272576400007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1138]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, LF,Wang, YG,Xiao, T,et al. Suppression of cancer growth in mice by adeno-associated virus vector-mediated IFN-beta expression driven by hTERT promoter[J]. CANCER LETTERS,2009,286(2):196-205.
APA	He, LF.,Wang, YG.,Xiao, T.,Zhang, KJ.,Li, GC.,...&Liu, XY.(2009).Suppression of cancer growth in mice by adeno-associated virus vector-mediated IFN-beta expression driven by hTERT promoter.CANCER LETTERS,286(2),196-205.
MLA	He, LF,et al."Suppression of cancer growth in mice by adeno-associated virus vector-mediated IFN-beta expression driven by hTERT promoter".CANCER LETTERS 286.2(2009):196-205.