Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E

	Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E
	Guo, CY; Diao, H; Lian, YD; Yu, HG; Gao, HC; Zhang, YL; Lin, DH
刊名	JOURNAL OF BIOTECHNOLOGY
	2009
卷号	139 期号:1 页码:33-37
关键词	BIN1b Size-exclusion CD HSQC Oligomerization Antimicrobial activity
通讯作者	Lin, DH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China.,dhlin@mail.shcnc.ac.cn
英文摘要	BIN1b was reported as all epididymis-specific beta-defensin antimicrobial peptide. In this paper. the recombinant BIN1b was expressed and purified by fusing with GB1-His tag. The size-exclusion gel filtration experiment indicated that the fusion protein GB1-BIN1b formed multimers at pH 7.4. and existed as monomer at pH 4.5. The oligomerization of GB1-BIN1b was only related to PH value, neither to NaCl concentration nor protein concentration. Far-UV circular dichroism (CD) spectra also showed the fusion protein had more ordered secondary structures at pH 4.5 than at pH 7.4,as a negative peak appeared around 218 nm indicative of typical beta-sheet. The 2D (15)N-(1)H heteronuclear single-quantum coherence (HSQC) spectra suggested that the fusion protein adopted a compact three-dimensional structure at pH 4.5. Colony following unit (CFU) inhibition assay demonstrated that 25 mu M fusion protein at pH 7.4 had all anti microbial activity of 40% against E. coli K(12)D(31). which might imply the fusion protein functions as multimeric states. In conclusion the GB1 fusion partner helps BIN1b form a stable homogenous conformation to facilitate subsequent structural determination Without a significant effect oil the antimicrobial activity. (c) 2008 Elsevier B.V. All rights reserved.
学科主题	Biotechnology & Applied Microbiology
类目[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	HIGH-LEVEL EXPRESSION ; ESCHERICHIA-COLI ; BETA-DEFENSIN ; PROTEIN ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000262795600006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1134]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, CY,Diao, H,Lian, YD,et al. Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E[J]. JOURNAL OF BIOTECHNOLOGY,2009,139(1):33-37.
APA	Guo, CY.,Diao, H.,Lian, YD.,Yu, HG.,Gao, HC.,...&Lin, DH.(2009).Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E.JOURNAL OF BIOTECHNOLOGY,139(1),33-37.
MLA	Guo, CY,et al."Recombinant expression and characterization of an epididymis-specific antimicrobial peptide BIN1b/SPAG11E".JOURNAL OF BIOTECHNOLOGY 139.1(2009):33-37.