Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method

	Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method
	Lu, H; Wen, JA; Wang, X; Yuan, K; Li, W; Lu, HB; Zhou, YL; Jin, KJ; Ruan, KC; Yang, GZ
刊名	JOURNAL OF OPTICS
	2010
卷号	12 期号:9 页码:95301-95301
关键词	oblique-incidence reflectivity difference protein microarrays label-free detection
通讯作者	Lu, H (reprint author), Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China.,kcruan@sibs.ac.cn ; yanggz@aphy.iphy.ac.cn
英文摘要	The specific binding between Cy5-labeled goat anti-mouse Immunoglobulin G (IgG) and mouse IgG with a concentration range from 625 to 10(4) mu g ml(-1) has been detected successfully by the oblique-incidence reflectivity difference (OI-RD) method in each procedure of microarray fabrication. The experimental data prove that the OI-RD method can be employed not only to distinguish the different concentrations in label-free fashion but also to detect the antibody-antigen capture. In addition, the differential treatment of the OI-RD signals can decrease the negative influences of glass slide as the microarray upholder. Therefore the OI-RD technique has promising applications for the label-free and high-throughput detection of protein microarrays.
学科主题	Optics
类目[WOS]	Optics
关键词[WOS]	PROTEOMICS
收录类别	SCI
语种	英语
WOS记录号	WOS:000282132900009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1042]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lu, H,Wen, JA,Wang, X,et al. Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method[J]. JOURNAL OF OPTICS,2010,12(9):95301-95301.
APA	Lu, H.,Wen, JA.,Wang, X.,Yuan, K.,Li, W.,...&Yang, GZ.(2010).Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method.JOURNAL OF OPTICS,12(9),95301-95301.
MLA	Lu, H,et al."Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method".JOURNAL OF OPTICS 12.9(2010):95301-95301.