Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method

	Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method
	Lu, H; Wen, JA; Wang, X; Yuan, K; Lu, HB; Zhou, YL; Jin, KJ; Yang, GZ; Li, W; Ruan, KC
刊名	SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
	2010
卷号	53 期号:7 页码:1230-1233
关键词	protein microarrays oblique-incidence reflectivity difference (OI-RD) label-free detection
通讯作者	Yang, GZ (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China.,yanggz@aphy.iphy.ac.cn ; kcruan@sibs.ac.cn
英文摘要	Mouse-Immunoglobulin G (mouse-IgG) with different concentrations in a range from 1000 to 0.0128 mu g/mL and a specific hybridization with goat anti-mouse IgG were detected successfully by using an oblique-incidence reflectivity difference (OI-RD) method. Two detection signals, consisting of an imaginary part (Im{Delta(p)-Delta(s)}) and a real part (Re{Delta(p)-Delta(s)}) of OI-RD, were obtained simultaneously. The detection results of hybridization by OI-RD were in accord with that of traditional fluorescent scans. In particular, we label-freely detected the washed mouse-IgG microarray with a series of concentrations and acquired a linear correlation between OI-RD intensities and the protein concentrations in logarithmic coordinates. The detection sensitivity of OI-RD can reach 14 fg. These experimental results suggest that the OI-RD method has potential applications in proteomics and clinical diagnosis.
学科主题	Physics
类目[WOS]	Physics, Multidisciplinary
收录类别	SCI
语种	英语
WOS记录号	WOS:000280210700012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1033]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Lu, H,Wen, JA,Wang, X,et al. Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2010,53(7):1230-1233.
APA	Lu, H.,Wen, JA.,Wang, X.,Yuan, K.,Lu, HB.,...&Ruan, KC.(2010).Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,53(7),1230-1233.
MLA	Lu, H,et al."Detection of hybridization of protein microarrays using an oblique-incidence reflectivity difference method".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 53.7(2010):1230-1233.