Two short D-Phe-containing cysteine-free conopeptides from Conus marmoreus

	Two short D-Phe-containing cysteine-free conopeptides from Conus marmoreus
	Zhang, L; Shao, XX; Chi, CW; Wang, CG
刊名	PEPTIDES
	2010
卷号	31 期号:1 页码:177-179
通讯作者	Chi, CW (reprint author), Tongji Univ, Inst Prot Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,zwqi@sibs.ac.cn ; chunguangwang@tongji.edu.cn
英文摘要	The cysteine-free conopeptides are naturally occurring components of the venom of cone snails and have been relatively less investigated than the cysteine-containing conopeptides. In this work, we used thiol-exchange chromatography to isolate cysteine-free conopeptides from the venom of Conus marmoreus. The full-length previously reported conomarphin and two novel shortened forms of it were found in the cysteine-free conopeptide fraction. The two shortened conomarphins also contain a D-Phe and a hydroxylated proline residue, suggesting complex post-translational modifications of these conopeptides. In particular, the shorter conomarphin fragment shares significant sequence similarity with conophans. This work demonstrated that thiol-exchange chromatography is a useful approach to isolate cysteine-free conopeptides from venom cocktail. The newly identified conopeptides further revealed the complexity of post-translational processing and the high diversity of conotoxins. (C) 2009 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Pharmacology & Pharmacy
类目[WOS]	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]	D-AMINO-ACID ; GAMMA-CARBOXYGLUTAMATE ; PEPTIDE ; VENOM
收录类别	SCI
语种	英语
WOS记录号	WOS:000274375500025
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/938]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, L,Shao, XX,Chi, CW,et al. Two short D-Phe-containing cysteine-free conopeptides from Conus marmoreus[J]. PEPTIDES,2010,31(1):177-179.
APA	Zhang, L,Shao, XX,Chi, CW,&Wang, CG.(2010).Two short D-Phe-containing cysteine-free conopeptides from Conus marmoreus.PEPTIDES,31(1),177-179.
MLA	Zhang, L,et al."Two short D-Phe-containing cysteine-free conopeptides from Conus marmoreus".PEPTIDES 31.1(2010):177-179.