The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells

	The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells
	Du, D; Xu, FL; Yu, LH; Zhang, CY; Lu, XF; Yuan, HX; Huang, Q; Zhang, F; Bao, HY; Jia, LH
刊名	DEVELOPMENTAL CELL
	2010
卷号	18 期号:1 页码:52-63
通讯作者	Chen, ZJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,zjchen@sibs.ac.cn
英文摘要	Cell polarity proteins regulate tight junction formation and directional migration in epithelial cells. To date, the mechanism by which these polarity proteins assemble at the leading edge of migrating epithelial cells remains unclear. We report that occludin, a transmembrane protein, is localized at the leading edge of migrating cells and regulates directional cell migration. During migration, occludin knockdown disrupted accumulation of aPKC-Par3 and PATJ at the leading edge, and led to a disorganized microtubule network and defective reorientation of the microtubule organization center (MTOC). Phosphorylation of occludin at tyrosine 473 residue allowed recruitment of p85 alpha to the leading edge via association with its C-terminal SH2 domain. Loss of occludin attenuated activation of PI3K, leading to disorganization of the actin cytoskeleton and reduced cell protrusions. Our data indicate that occludin is required for the leading-edge localization of polarity proteins aPKC-Par3 and PATJ and promotes cell protrusion by regulating membrane-localized activation of PI3K.
学科主题	Cell Biology ; Developmental Biology
类目[WOS]	Cell Biology ; Developmental Biology
关键词[WOS]	DISTINCT ROLES ; RHO-GTPASES ; TYROSINE PHOSPHORYLATION ; MEMBRANE-PROTEIN ; OXIDATIVE STRESS ; LEADING-EDGE ; POLARITY ; CDC42 ; POLARIZATION ; COMPLEX
收录类别	SCI
语种	英语
WOS记录号	WOS:000273829800008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/922]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Du, D,Xu, FL,Yu, LH,et al. The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells[J]. DEVELOPMENTAL CELL,2010,18(1):52-63.
APA	Du, D.,Xu, FL.,Yu, LH.,Zhang, CY.,Lu, XF.,...&Chen, ZJ.(2010).The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells.DEVELOPMENTAL CELL,18(1),52-63.
MLA	Du, D,et al."The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells".DEVELOPMENTAL CELL 18.1(2010):52-63.