Histone H3 Lysine 4 Hypermethylation Prevents Aberrant Nucleosome Remodeling at the PHO5 Promoter

	Histone H3 Lysine 4 Hypermethylation Prevents Aberrant Nucleosome Remodeling at the PHO5 Promoter
	Wang, SS; Zhou, BO; Zhou, JQ
刊名	MOLECULAR AND CELLULAR BIOLOGY
	2011
卷号	31 期号:15 页码:3171-3181
通讯作者	Zhou, BO (reprint author), Chinese Acad Sci, Grad Sch, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,zhoub@sibs.ac.cn ; jqzhou@sibs.ac.cn
英文摘要	Recent studies have highlighted the histone H3K4 methylation (H3K4me)-dependent transcriptional repression in Saccharomyces cerevisiae; however, the underlying mechanism remains inexplicit. Here, we report that H3K4me inhibits the basal PHO5 transcription under high-phosphate conditions by suppressing nucleosome disassembly at the promoter. We found that derepression of the PHO5 promoter by SET1 deletion resulted in a labile chromatin structure, allowing more binding of RNA polymerase II (Pol II) but not the transactivators Pho2 and Pho4. We further showed that Pho23 and Cti6, two plant homeodomain (PHD)-containing proteins, cooperatively anchored the large Rpd3 (Rpd3L) complex to the H3K4-methylated PHO5 promoter. The deacetylation activity of Rpd3 on histone H3 was required for the function of Set1 at the PHO5 promoter. Taken together, our data suggest that Set1-mediated H3K4me suppresses nucleosome remodeling at the PHO5 promoter so as to reduce basal transcription of PHO5 under repressive conditions. We propose that the restriction of aberrant nucleosome remodeling contributes to strict control of gene transcription by the transactivators.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	RNA-POLYMERASE-II ; SACCHAROMYCES-CEREVISIAE ; YEAST PHO5 ; DEACETYLASE COMPLEX ; IN-VIVO ; TRANSCRIPTIONAL ACTIVATION ; GENOME-WIDE ; CHROMATIN MODIFICATIONS ; POSITIONED NUCLEOSOMES ; DISTINCT FUNCTIONS
收录类别	SCI
语种	英语
WOS记录号	WOS:000292595300010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/877]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, SS,Zhou, BO,Zhou, JQ. Histone H3 Lysine 4 Hypermethylation Prevents Aberrant Nucleosome Remodeling at the PHO5 Promoter[J]. MOLECULAR AND CELLULAR BIOLOGY,2011,31(15):3171-3181.
APA	Wang, SS,Zhou, BO,&Zhou, JQ.(2011).Histone H3 Lysine 4 Hypermethylation Prevents Aberrant Nucleosome Remodeling at the PHO5 Promoter.MOLECULAR AND CELLULAR BIOLOGY,31(15),3171-3181.
MLA	Wang, SS,et al."Histone H3 Lysine 4 Hypermethylation Prevents Aberrant Nucleosome Remodeling at the PHO5 Promoter".MOLECULAR AND CELLULAR BIOLOGY 31.15(2011):3171-3181.