Human cell-death-inducing DFF45-like effector C induces apoptosis via caspase-8

	Human cell-death-inducing DFF45-like effector C induces apoptosis via caspase-8
	Tang, X; Xing, Z; Tang, H; Liang, L; Zhao, MJ
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2011
卷号	43 期号:10 页码:779-786
关键词	apoptosis CIDEC DNA fragmentation caspases FADD
通讯作者	Zhao, MJ (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn
英文摘要	Human cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector C (CIDEC) is a potent apoptotic inducer. Previous studies have indicated that the Fat-specific protein 27 (Fsp27), a mouse homolog of CIDEC, induces apoptosis via caspase-3, -7, and -9 and triggers the release of cytochrome c from mitochondria, which implies that the mitochondrial pathway is involved in Fsp27-induced apoptosis. In the current study, we found that CIDEC-induced apoptosis was mediated by caspase-8. The caspase inhibitor assay showed that CIDEC-induced apoptosis was dramatically reduced in the presence of the general caspase inhibitor, the caspase-3 inhibitor, and the caspase-8 inhibitor, whereas the caspase-9 inhibitor only weakly inhibited CIDEC-induced apoptosis. These results confirmed that the activation of caspase-3 and caspase-8 were involved in CIDEC-induced apoptosis. Moreover, in caspase-3- or caspase-8-deficient cells, CIDEC-induced apoptosis were dramatically decreased, which demonstrated that CIDEC-induced apoptosis might require the activation of caspase-3 and caspase-8. Because caspase-8 in general is a key effecter of death-receptor pathway and activated by Fas-Associated protein with Death Domain (FADD), we examined whether FADD was involved in CIDEC-induced apoptosis. Our results demonstrated that CIDEC-induced apoptosis was independent of FADD, suggesting that CIDEC-induced apoptosis might be in a death-receptor-independent, caspase-8-dependent manner. It was also found that the region of amino acid 168-200 in carboxyl domain of CIDEC was critical for its crucial pro-apoptotic function.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	DNA-FRAGMENTATION-FACTOR ; DEPENDENT APOPTOSIS ; PROTEIN ; FAS ; ACTIVATION ; EXPRESSION ; RECEPTORS ; PROTEASES ; PATHWAY ; FAMILY
收录类别	SCI
语种	英语
WOS记录号	WOS:000295411300005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/858]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Tang, X,Xing, Z,Tang, H,et al. Human cell-death-inducing DFF45-like effector C induces apoptosis via caspase-8[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(10):779-786.
APA	Tang, X,Xing, Z,Tang, H,Liang, L,&Zhao, MJ.(2011).Human cell-death-inducing DFF45-like effector C induces apoptosis via caspase-8.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(10),779-786.
MLA	Tang, X,et al."Human cell-death-inducing DFF45-like effector C induces apoptosis via caspase-8".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.10(2011):779-786.