Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation

	Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation
	Li, ZF; Nie, F; Wang, S; Li, L
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2011
卷号	108 期号:8 页码:3116-3123
关键词	epigenetic regulation zebrafish embryonic development
通讯作者	Li, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,lli@sibs.ac.cn
英文摘要	Histone methylation has an important role in transcriptional regulation. However, unlike H3K4 and H3K9 methylation, the role of H4K20 monomethylation (H4K20me-1) in transcriptional regulation remains unclear. Here, we show that Wnt3a specifically stimulates H4K20 monomethylation at the T cell factor (TCF)-binding element through the histone methylase SET8. Additionally, SET8 is crucial for activation of the Wnt reporter gene and target genes in both mammalian cells and zebrafish. Furthermore, SET8 interacts with lymphoid enhancing factor-1 (LEF1)/TCF4 directly, and this interaction is regulated by Wnt3a. Therefore, we conclude that SET8 is a Wnt signaling mediator and is recruited by LEF1/TCF4 to regulate the transcription of Wnt-activated genes, possibly through H4K20 monomethylation at the target gene promoters. Our findings also indicate that H4K20me-1 is a marker for gene transcription activation, at least in canonical Wnt signaling.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	BETA-CATENIN ; TRANSCRIPTION ACTIVATION ; DNA-REPLICATION ; S-PHASE ; C-JUN ; CHROMATIN ; METHYLTRANSFERASE ; COMPLEX ; LYSINE-20 ; DISEASE
收录类别	SCI
语种	英语
WOS记录号	WOS:000287580400013
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/846]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, ZF,Nie, F,Wang, S,et al. Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2011,108(8):3116-3123.
APA	Li, ZF,Nie, F,Wang, S,&Li, L.(2011).Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,108(8),3116-3123.
MLA	Li, ZF,et al."Histone H4 Lys 20 monomethylation by histone methylase SET8 mediates Wnt target gene activation".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108.8(2011):3116-3123.