Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3

	Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3
	Gao, XC; Zhou, CJ; Zhou, ZR; Zhang, YH; Zheng, XM; Song, AX; Hu, HY
刊名	PLOS ONE
	2011
卷号	6 期号:5 页码:e19763-e19763
通讯作者	Gao, XC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China.,hyhu@sibs.ac.cn
英文摘要	Homo sapiens J domain protein (HSJ1) is a J-domain containing co-chaperone that is known to stimulate ATPase activity of HSP70 chaperone, while it also harbors two ubiquitin (Ub)-interacting motifs (UIMs) that may bind with ubiquitinated substrates and potentially function in protein degradation. We studied the effects of HSJ1a on the protein levels of both normal and the disease-related polyQ-expanded forms of ataxin-3 (Atx3) in cells. The results demonstrate that the N-terminal J-domain and the C-terminal UIM domain of HSJ1a exert opposite functions in regulating the protein level of cellular overexpressed Atx3. This dual regulation is dependent on the binding of the J-domain with HSP70, and the UIM domain with polyUb chains. The J-domain down-regulates the protein level of Atx3 through HSP70 mediated proteasomal degradation, while the UIM domain may alleviate this process via maintaining the ubiquitinated Atx3. We propose that cochaperone HSJ1a orchestrates the balance of substrates in stressed cells in a Yin-Yang manner.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	HEAT-SHOCK-PROTEIN ; UBIQUITIN-DEPENDENT DEGRADATION ; MACHADO-JOSEPH-DISEASE ; NITRIC-OXIDE SYNTHASE ; HSP70 ATPASE ACTIVITY ; MOLECULAR CHAPERONES ; POLYGLUTAMINE DISEASE ; MISFOLDING DISEASES ; SUBSTRATE-BINDING ; QUALITY-CONTROL
收录类别	SCI
语种	英语
WOS记录号	WOS:000290771200020
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/793]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, XC,Zhou, CJ,Zhou, ZR,et al. Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3[J]. PLOS ONE,2011,6(5):e19763-e19763.
APA	Gao, XC.,Zhou, CJ.,Zhou, ZR.,Zhang, YH.,Zheng, XM.,...&Hu, HY.(2011).Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3.PLOS ONE,6(5),e19763-e19763.
MLA	Gao, XC,et al."Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3".PLOS ONE 6.5(2011):e19763-e19763.