TNF alpha-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors

	TNF alpha-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors
	Liu, SH; Yang, Y; Wu, JR
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2011
卷号	414 期号:3 页码:618-624
关键词	TNF alpha MicroRNA 3T3-L1 cell Adipogenesis
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn
英文摘要	Tumor necrosis factor alpha (TNF alpha) is known to inhibit adipogenesis, but the molecular mechanism of this inhibition remains elusive. In the present study, we found that TNF alpha-induced inhibition of adipogenesis mainly occurs when 3T3-L1 preadipocytes are treated with TNF alpha within 2 h induction of adipogenesis. We revealed that TNF alpha treatment results in the up-regulation of miR-155 through the NF kappa B pathway in 3T3-L1 cells. This overexpression of miR-155 may suppress the expression of C/EBP beta and CREB by directly targeting their 3' untranslated regions (3' UTRs). Importantly, anti-miR-155 reduces the TNF alpha-induced inhibition of adipogenesis, whereas exogenous expression of mir-155 inhibits adipogenesis. Taken together, these findings reveal a novel role for TNF alpha in the regulation of anti-adipogenic miRNAs. (C) 2011 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	NECROSIS-FACTOR-ALPHA ; NF-KAPPA-B ; 3T3-L1 ADIPOCYTE DIFFERENTIATION ; BINDING PROTEIN BETA ; C/EBP-BETA ; GENE-EXPRESSION ; CELLS ; LIPOLYSIS ; MICE ; MICRORNA-155
收录类别	SCI
语种	英语
WOS记录号	WOS:000298519500030
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/760]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, SH,Yang, Y,Wu, JR. TNF alpha-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,414(3):618-624.
APA	Liu, SH,Yang, Y,&Wu, JR.(2011).TNF alpha-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,414(3),618-624.
MLA	Liu, SH,et al."TNF alpha-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 414.3(2011):618-624.