Ultra-high resolution crystal structure of a dimeric defensin SPE10

	Ultra-high resolution crystal structure of a dimeric defensin SPE10
	Song, XM; Zhang, M; Zhou, ZC; Gong, WM
刊名	FEBS LETTERS
	2011
卷号	585 期号:2 页码:300-306
关键词	Defensin Crystal structure Ultra-high resolution Mechanism Dimer
通讯作者	Zhou, ZC (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zczhou@sibs.ac.cn ; wgong@sun5.ibp.ac.cn
英文摘要	Defensins are key players of the innate immune system known to act against bacteria, fungi and viruses. Here we report the 0.98-angstrom crystal structure of SPE10, a dimeric plant defensin. SPE10 associates as a dimer through a unique amino acid triplet involving residues R36-W42-R40. The helix from one subunit interacts with arginines R36 and R40 from the other subunit, forming a sheet-like dimer with a highly extended molecular surface. A conserved hydrophobic patch on the molecular head largely overlaps with the putative receptor-binding site previously reported for another defensin. Structural analysis and mutational studies indicate that the dimeric association of SPE10 is relevant to its function, and that the hydrophobic patch on the molecular head is required for its antifungal activity. Structured summary: SPE10 binds to SPE10 by X-ray crystallography (View interaction) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	3-DIMENSIONAL SOLUTION STRUCTURE ; NUCLEAR-MAGNETIC-RESONANCE ; PACHYRRHIZUS-EROSUS SEEDS ; PLANT ANTIFUNGAL PROTEINS ; ANTIMICROBIAL PEPTIDES ; DNA-RECOGNITION ; FUNGAL GROWTH ; HOST-DEFENSE ; MEMBRANES ; COMPLEX
收录类别	SCI
语种	英语
WOS记录号	WOS:000286180300007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/756]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Song, XM,Zhang, M,Zhou, ZC,et al. Ultra-high resolution crystal structure of a dimeric defensin SPE10[J]. FEBS LETTERS,2011,585(2):300-306.
APA	Song, XM,Zhang, M,Zhou, ZC,&Gong, WM.(2011).Ultra-high resolution crystal structure of a dimeric defensin SPE10.FEBS LETTERS,585(2),300-306.
MLA	Song, XM,et al."Ultra-high resolution crystal structure of a dimeric defensin SPE10".FEBS LETTERS 585.2(2011):300-306.