A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms

	A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms
	Chen, Y; Rai, R; Zhou, ZR; Kanoh, J; Ribeyre, C; Yang, YT; Zheng, H; Damay, P; Wang, F; Tsujii, H
刊名	NATURE STRUCTURAL & MOLECULAR BIOLOGY
	2011
卷号	18 期号:2 页码:213-U282
通讯作者	Lei, M (reprint author), Univ Michigan, Sch Med, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA.,s.chang@yale.edu ; leim@umich.edu
英文摘要	Repressor activator protein 1 (RAP1) is the most highly conserved telomere protein. It is involved in protecting chromosome ends in fission yeast and promoting gene silencing in Saccharomyces cerevisiae, whereas it represses homology-directed recombination at telomeres in mammals. To understand how RAP1 has such diverse functions at telomeres, we solved the crystal or solution structures of the RAP1 C-terminal (RCT) domains of RAP1 from multiple organisms in complex with their respective protein-binding partners. Our analysis establishes RAP1(RCT) as an evolutionarily conserved protein-protein interaction module. In mammalian and fission yeast cells, this module interacts with TRF2 and Taz1, respectively, targeting RAP1 to chromosome ends for telomere protection. In contrast, S. cerevisiae RAP1 uses its RCT domain to recruit Sir3 to telomeres to mediate gene silencing. Together, our results show that, depending on the organism, the evolutionarily conserved RAP1 RCT motif has diverse functional roles at telomeres.
学科主题	Biochemistry & Molecular Biology; Biophysics; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]	NMR STRUCTURE CALCULATION ; SINGLE-STRANDED-DNA ; SACCHAROMYCES-CEREVISIAE ; FISSION YEAST ; SCHIZOSACCHAROMYCES-POMBE ; HOMOLOGOUS RECOMBINATION ; DYSFUNCTIONAL TELOMERES ; CRYSTAL-STRUCTURE ; DAMAGE RESPONSE ; NOE ASSIGNMENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000286969200015
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/752]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chen, Y,Rai, R,Zhou, ZR,et al. A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2011,18(2):213-U282.
APA	Chen, Y.,Rai, R.,Zhou, ZR.,Kanoh, J.,Ribeyre, C.,...&Lei, M.(2011).A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms.NATURE STRUCTURAL & MOLECULAR BIOLOGY,18(2),213-U282.
MLA	Chen, Y,et al."A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms".NATURE STRUCTURAL & MOLECULAR BIOLOGY 18.2(2011):213-U282.