Kruppel-like factor KLF9 regulates PPAR gamma transactivation at the middle stage of adipogenesis

	Kruppel-like factor KLF9 regulates PPAR gamma transactivation at the middle stage of adipogenesis
	Pei, H; Yao, Y; Yang, Y; Liao, K; Wu, JR
刊名	CELL DEATH AND DIFFERENTIATION
	2011
卷号	18 期号:2 页码:315-327
关键词	KLF9 adipogenesis PPAR gamma 2 C/EBP alpha 3T3-L1 cell
通讯作者	Wu, JR (reprint author), Chinese Acad Sci, Key Lab Syst Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,kliao@sibs.ac.cn ; wujr@sibs.ac.cn
英文摘要	Kruppel-like factors (KLFs) as a family of zinc-finger transcription factors involve in the regulation of many physiological processes. In these studies, KLF9 was characterized for its role in adipogenesis. The expression of KLF9 was markedly upregulated during the middle stage of 3T3-L1 adipocyte differentiation, and inhibition of KLF9 by RNAi impaired adipogenesis. Using promoter deletion and mutation analysis, we identified two KLF9-binding sites within the 0.6-kb region of the PPAR gamma 2 proximal promoter, indicating that KLF9 interacts with the PPAR gamma 2 promoter. Furthermore, we found that KLF9 could synergistically activate PPAR gamma 2 promoter by directly interacting with C/EBP alpha. In addition, overexpression of PPAR gamma 2 rescued the impairment of adipocyte differentiation induced by KLF9 knockdown, which supports that PPAR gamma 2 is a downstream target of KLF9. Collectively, our results indicate KLF9 as a key pro-adipogenic transcription factor through regulation of PPAR gamma 2 expression with C/EBP alpha at the middle stage of adipogenesis. Cell Death and Differentiation (2011) 18, 315-327; doi: 10.1038/cdd.2010.100; published online 20 August 2010
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	ADIPOCYTE DIFFERENTIATION ; TRANSCRIPTIONAL REGULATION ; ADIPOSE CONVERSION ; MICE LACKING ; IN-VIVO ; GENE ; BINDING ; PROMOTER ; EXPRESSION ; PROTEIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000286045800012
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/749]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Pei, H,Yao, Y,Yang, Y,et al. Kruppel-like factor KLF9 regulates PPAR gamma transactivation at the middle stage of adipogenesis[J]. CELL DEATH AND DIFFERENTIATION,2011,18(2):315-327.
APA	Pei, H,Yao, Y,Yang, Y,Liao, K,&Wu, JR.(2011).Kruppel-like factor KLF9 regulates PPAR gamma transactivation at the middle stage of adipogenesis.CELL DEATH AND DIFFERENTIATION,18(2),315-327.
MLA	Pei, H,et al."Kruppel-like factor KLF9 regulates PPAR gamma transactivation at the middle stage of adipogenesis".CELL DEATH AND DIFFERENTIATION 18.2(2011):315-327.