Positive selection-guided mutational analysis revealing two key functional sites of scorpion ERG K+ channel toxins

	Positive selection-guided mutational analysis revealing two key functional sites of scorpion ERG K+ channel toxins
	Wang, XL; Jimenez-Vargas, JM; Xu, CQ; Possani, LD; Zhu, SY
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2012
卷号	429 期号:1 页码:111-116
关键词	Adaptive evolution hERG channels Maximum likelihood Mutational experiments Scorpion toxin
通讯作者	Possani, LD (reprint author), Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Med & Bioproc, Av Univ 2001,POB 510-3, Cuernavaca 62210, Morelos, Mexico.,possani@ibt.unam.mx ; Zhusy@ioz.ac.cn
英文摘要	Scorpion gamma-KTx toxins are important molecular tools for studying physiological and pharmacological functions of human ether-a-go-go related gene (hERG) K+ channels. To pinpoint functional residues of this class of toxins involved in channel binding, we employed a combined approach that integrates evolutionary information and site-directed mutagenesis. Among three positively selected sites (PSSs) identified here, two (Gln18 and Met35) were found to be associated with the toxin's function because their changes significantly decreased the potency of ErgTx1 (also called CnErg1) on hERG1 channel. On the contrary, no potency alteration was observed at the third PSS (Ala42) when the mutation was introduced, which could be due to its location far from the functional surface of the toxin. Our strategy will accelerate the research of structure-function relationship of scorpion K+ channel toxins. (C) 2012 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	CODON-SUBSTITUTION MODELS ; AMINO-ACID SITES ; ADAPTIVE EVOLUTION ; MAXIMUM-LIKELIHOOD ; MECHANISM ; GENETICS ; CNERG1 ; BLOCK ; VENOM
收录类别	SCI
语种	英语
WOS记录号	WOS:000312513700019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/697]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Wang, XL,Jimenez-Vargas, JM,Xu, CQ,et al. Positive selection-guided mutational analysis revealing two key functional sites of scorpion ERG K+ channel toxins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,429(1):111-116.
APA	Wang, XL,Jimenez-Vargas, JM,Xu, CQ,Possani, LD,&Zhu, SY.(2012).Positive selection-guided mutational analysis revealing two key functional sites of scorpion ERG K+ channel toxins.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,429(1),111-116.
MLA	Wang, XL,et al."Positive selection-guided mutational analysis revealing two key functional sites of scorpion ERG K+ channel toxins".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 429.1(2012):111-116.