MicroRNA-143 (miR-143) Regulates Cancer Glycolysis via Targeting Hexokinase 2 Gene

	MicroRNA-143 (miR-143) Regulates Cancer Glycolysis via Targeting Hexokinase 2 Gene
	Fang, R; Xiao, T; Fang, ZY; Sun, YH; Li, F; Gao, YJ; Feng, Y; Li, L; Wang, Y; Liu, XL
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2012
卷号	287 期号:27 页码:23227-23235
通讯作者	Xiao, T (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,txiao@sibs.ac.cn ; xyliu@sibs.ac.cn ; hbji@sibs.ac.cn
英文摘要	High glycolysis, well known as "Warburg effect," is frequently observed in a variety of cancers. Whether the deregulation of miRNAs contributes to the Warburg effect remains largely unknown. Because miRNA regulates gene expression at both mRNA and protein levels, we constructed a gene functional association network, which allows us to detect the gene activity instead of gene expression, to integratively analyze the microarray data for gene expression and miRNA expression profiling and identify glycolysis-related gene-miRNA pairs deregulated in cancer. Hexokinase 2 (HK2), coding for the first rate-limiting enzyme of glycolysis, is among the top list of genes predicted and potentially regulated by multiple miRNAs including miR-143. Interestingly, miR-143 expression was inversely associated with HK2 protein level but not mRNA level in human lung cancer samples. miR-143, down-regulated by mammalian target of rapamycin activation, reduces glucose metabolism and inhibits cancer cell proliferation and tumor formation through targeting HK2. Collectively, we have not only established a novel methodology for gene-miRNA pair prediction but also identified miR-143 as an essential regulator of cancer glycolysis via targeting HK2.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	PROBE LEVEL DATA ; PROTEIN-SYNTHESIS ; LUNG-CANCER ; THERAPY ; WARBURG ; CELLS ; NORMALIZATION ; MITOCHONDRIA ; METABOLISM ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000306495000077
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/690]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Fang, R,Xiao, T,Fang, ZY,et al. MicroRNA-143 (miR-143) Regulates Cancer Glycolysis via Targeting Hexokinase 2 Gene[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(27):23227-23235.
APA	Fang, R.,Xiao, T.,Fang, ZY.,Sun, YH.,Li, F.,...&Ji, HB.(2012).MicroRNA-143 (miR-143) Regulates Cancer Glycolysis via Targeting Hexokinase 2 Gene.JOURNAL OF BIOLOGICAL CHEMISTRY,287(27),23227-23235.
MLA	Fang, R,et al."MicroRNA-143 (miR-143) Regulates Cancer Glycolysis via Targeting Hexokinase 2 Gene".JOURNAL OF BIOLOGICAL CHEMISTRY 287.27(2012):23227-23235.