Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord

	Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord
	Guo, Z; Zhao, CL; Huang, MG; Huang, TW; Fan, MR; Xie, ZQ; Chen, Y; Zhao, XL; Xia, GN; Geng, JL
刊名	JOURNAL OF NEUROSCIENCE
	2012
卷号	32 期号:25 页码:8509-8520
通讯作者	Cheng, LP (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lpcheng@ion.ac.cn
英文摘要	Establishing the pattern of expression of transmitters and peptides as well as their receptors in different neuronal types is crucial for understanding the circuitry in various regions of the brain. Previous studies have demonstrated that the transmitter and peptide phenotypes in mouse dorsal spinal cord neurons are determined by the transcription factors Tlx1/3 and Ptf1a. Here we show that these transcription factors also determine the expression of two distinct sets of transmitter and peptide receptor genes in this region. We have screened the expression of 78 receptor genes in the spinal dorsal horn by in situ hybridization. We found that receptor genes Gabra1, Gabra5, Gabrb2, Gria3, Grin3a, Grin3b, Galr1, and Npy1r were preferentially expressed in Tlx3-expressing glutamatergic neurons and their derivatives, and deletion of Tlx1 and Tlx3 resulted in the loss of expression of these receptor genes. Furthermore, we obtained genetic evidence that Tlx3 uses distinct pathways to control the expression of receptor genes. We also found that receptor genes Grm3, Grm4, Grm5, Grik1, Grik2, Grik3, and Sstr2 were mainly expressed in Pax2-expressing GABAergic neurons in the spinal dorsal horn, and their expression in this region was abolished or markedly reduced in Ptf1a and Pax2 deletion mutant mice. Together, our studies indicate that Tlx1/3 and Ptf1a, the key transcription factors for fate determination of glutamatergic and GABAergic neurons in the dorsal spinal cord, are also responsible for controlling the expression of two distinct sets of transmitter and peptide receptor genes.
学科主题	Neurosciences & Neurology
类目[WOS]	Neurosciences
关键词[WOS]	IONOTROPIC GLUTAMATE RECEPTORS ; NEUROPEPTIDE YY1 RECEPTOR ; LAMINAE-I-III ; GABAERGIC NEURONS ; SYNAPTIC-TRANSMISSION ; MOLECULAR-MECHANISMS ; INTERNATIONAL UNION ; SELECTOR GENE ; Y-RECEPTOR ; CELL FATE
收录类别	SCI
语种	英语
WOS记录号	WOS:000305678500010
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/689]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Guo, Z,Zhao, CL,Huang, MG,et al. Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord[J]. JOURNAL OF NEUROSCIENCE,2012,32(25):8509-8520.
APA	Guo, Z.,Zhao, CL.,Huang, MG.,Huang, TW.,Fan, MR.,...&Cheng, LP.(2012).Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord.JOURNAL OF NEUROSCIENCE,32(25),8509-8520.
MLA	Guo, Z,et al."Tlx1/3 and Ptf1a Control the Expression of Distinct Sets of Transmitter and Peptide Receptor Genes in the Developing Dorsal Spinal Cord".JOURNAL OF NEUROSCIENCE 32.25(2012):8509-8520.