Region-specific localization of IMDS-60 protein in mouse epididymis and its relationship with sperm maturation

	Region-specific localization of IMDS-60 protein in mouse epididymis and its relationship with sperm maturation
	Zhan, XN; Wang, CM; Liu, AH; Liu, Q; Zhang, YL
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2012
卷号	44 期号:11 页码:924-930
关键词	epididymis sperm maturation IMDS-60
通讯作者	Liu, Q (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China.,qliu@sibs.ac.cn ; ylzhang@sibs.ac.cn
英文摘要	Spermatozoa acquire forward motility and fertilizing capacity during their transit through the epididymis. This maturation process involves modifications of the sperm surface by different proteins secreted by a series of specialized regions in the epididymal epithelium. Previously, our lab has reported IMDS-60 gene, which is highly expressed in mouse corpus and cauda epididymidis. Here, to perform further characterization of IMDS-60 protein, the specific polyclonal antisera were raised to the C-terminal peptide of the IMDS-60 protein. Western blot and immunohistochemistry assay revealed that IMDS-60 protein was specifically localized in the corpus and cauda regions of epididymidis. IMDS-60 could be transported from epididymal epithelium to the surface of epididymal spermatozoa. Finally, the results of the antibody block experiments suggested that IMDS-60 might play important roles in sperm maturation in the epididymis.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	GPI-ANCHORED PROTEINS ; SACCHAROMYCES-CEREVISIAE ; BIOSYNTHESIS ; CAPACITATION ; SPERMATOZOA ; PATHWAY ; CWH43 ; SPAM1 ; CD52 ; HE5
收录类别	SCI
语种	英语
WOS记录号	WOS:000310370800005
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/659]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhan, XN,Wang, CM,Liu, AH,et al. Region-specific localization of IMDS-60 protein in mouse epididymis and its relationship with sperm maturation[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(11):924-930.
APA	Zhan, XN,Wang, CM,Liu, AH,Liu, Q,&Zhang, YL.(2012).Region-specific localization of IMDS-60 protein in mouse epididymis and its relationship with sperm maturation.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(11),924-930.
MLA	Zhan, XN,et al."Region-specific localization of IMDS-60 protein in mouse epididymis and its relationship with sperm maturation".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.11(2012):924-930.