Secretome profile of mouse oocytes after activation using mass spectrum

	Secretome profile of mouse oocytes after activation using mass spectrum
	Peng, QP; Yang, H; Xue, SG; Shi, LY; Yu, Q; Kuang, YP
刊名	JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
	2012
卷号	29 期号:8 页码:765-771
关键词	Oocyte Secretome Activation Mass spectrometry
通讯作者	Xue, SG (reprint author), Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Assisted Reprod, Shanghai 200011, Peoples R China.,immunxsg@yahoo.com.cn ; kuangyanp@126.com
英文摘要	Mammalian oocytes undergo a cortical reaction after fertilization, releasing cortical granules and other proteins into the perivitelline space and inhibiting polyspermy. Few studies have evaluated the biological functions and properties of these proteins. We investigated mouse oocytes in which the zona pellucida (ZP) was present (ZP-intact group) or absent (ZP-free group). After being activated by Srcl2, secreted proteins are collected from mouse oocytes. Mass spectrometry analysis was performed that identified proteins such as Ldhb, PADi6, Uchl1, Pebp1, Alb, Hsp90aa1, Prss1, trypsinogen 7, trypsin 4, trypsin 10, Sod1, Zp1, Zp2, Zp3, Akap8, Npm2, Pkm2 and Ppia in the ZP-free group. Proteins such as Ldhb, Uchl1, Prss1, trypsin 10, trypsinogen 7, and Ast1 were identified in the ZP-intact groups. The expression of some proteins, including Ldhb, Alb and Sod1, were initially detected following oocyte activation. The finding of four trypsin subtypes, such as Prss1, further support previous observations. Studies investigating the physiological functions and properties of these proteins are ongoing. Research on these cortical proteins provides a theoretical basis for understanding polyspermy inhibition at the level of ZP and gives technological support for fertilization detection, assessment of oocyte quality and embryonic culture.
学科主题	Genetics & Heredity; Obstetrics & Gynecology; Reproductive Biology
类目[WOS]	Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]	CORTICAL GRANULE ENVELOPE ; IN-VITRO ; PLASMA-MEMBRANE ; ZONA REACTION ; PROTEIN ; EGGS ; FERTILIZATION ; LOCALIZATION ; EXPRESSION ; EMBRYOS
收录类别	SCI
语种	英语
WOS记录号	WOS:000308184900009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/629]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Peng, QP,Yang, H,Xue, SG,et al. Secretome profile of mouse oocytes after activation using mass spectrum[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2012,29(8):765-771.
APA	Peng, QP,Yang, H,Xue, SG,Shi, LY,Yu, Q,&Kuang, YP.(2012).Secretome profile of mouse oocytes after activation using mass spectrum.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,29(8),765-771.
MLA	Peng, QP,et al."Secretome profile of mouse oocytes after activation using mass spectrum".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 29.8(2012):765-771.