Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit

	Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit
	Huang, Y; Li, WC; Yao, X; Lin, QJ; Yin, JW; Liang, Y; Heiner, M; Tian, B; Hui, JY; Wang, G
刊名	MOLECULAR CELL
	2012
卷号	45 期号:4 页码:459-469
通讯作者	Wang, G (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,gwang22@sibs.ac.cn
英文摘要	Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its MED23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recombinant Mediator subunit, MED23, but not to several other Mediator components. One of these factors, hnRNP L, specifically interacts with MED23 in vitro and in vivo. Consistently, Mediator partially colocalizes with hnRNP L and the splicing machinery in the cell. Functionally, MED23 regulates a subset of hnRNP L-targeted alternative splicing (AS) and alternative cleavage and polyadenylation (APA) events, as shown by minigene reporters and exon array analysis. ChIP-seq analysis revealed that MED23 can regulate hnRNP L occupancy at their coregulated genes. Taken together, these results demonstrate a crosstalk between Mediator and the splicing machinery, providing a molecular basis for coupling mRNA processing to transcription.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	HNRNP-L ; GENE-EXPRESSION ; YEAST MEDIATOR ; IN-VIVO ; TRANSCRIPTION ; BINDING ; LINKS ; POLYADENYLATION ; RECRUITMENT ; ELONGATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000300753800006
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/585]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Huang, Y,Li, WC,Yao, X,et al. Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit[J]. MOLECULAR CELL,2012,45(4):459-469.
APA	Huang, Y.,Li, WC.,Yao, X.,Lin, QJ.,Yin, JW.,...&Wang, G.(2012).Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit.MOLECULAR CELL,45(4),459-469.
MLA	Huang, Y,et al."Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit".MOLECULAR CELL 45.4(2012):459-469.