Prevent cervical cancer by screening with reliable human papillomavirus detection and genotyping

	Prevent cervical cancer by screening with reliable human papillomavirus detection and genotyping
	Ge, SC; Gong, B; Cai, XS; Yang, XE; Gan, XW; Tong, XH; Li, HC; Zhu, MJ; Yang, FY; Zhou, HR
刊名	CANCER MEDICINE
	2012
卷号	1 期号:1 页码:59-67
关键词	Cervical cancer HPV prevention screen
通讯作者	Hong, GF (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,gfhong@sibs.ac.cn
英文摘要	The incidence of cervical cancer is expected to rise sharply in China. A reliable routine human papillomavirus (HPV) detection and genotyping test to be supplemented by the limited Papanicolaou cytology facilities is urgently needed to help identify the patients with cervical precancer for preventive interventions. To this end, we evaluated a nested polymerase chain reaction (PCR) protocol for detection of HPV L1 gene DNA in cervicovaginal cells. The PCR amplicons were genotyped by direct DNA sequencing. In parallel, split samples were subjected to a Digene HC2 HPV test which has been widely used for " cervical cancer risk" screen. Of the 1826 specimens, 1655 contained sufficient materials for analysis and 657 were truly negative. PCR/DNA sequencing showed 674 infected by a single high-risk HPV, 188 by a single low-risk HPV, and 136 by multiple HPV genotypes with up to five HPV genotypes in one specimen. In comparison, the HC2 test classified 713 specimens as infected by high-risk HPV, and 942 as negative for HPV infections. The high-risk HC2 test correctly detected 388 (57.6%) of the 674 high-risk HPV isolates in clinical specimens, mislabeled 88 (46.8%) of the 188 low-risk HPV isolates as high-risk genotypes, and classified 180 (27.4%) of the 657 " true-negative" samples as being infected by high-risk HPV. It was found to cross-react with 20 low-risk HPV genotypes. We conclude that nested PCR detection of HPV followed by short target DNA sequencing can be used for screening and genotyping to formulate a paradigm in clinical management of HPV-related disorders in a rapidly developing economy.
学科主题	Oncology
类目[WOS]	Oncology
收录类别	SCI
语种	英语
WOS记录号	WOS:000209210200008
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/529]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ge, SC,Gong, B,Cai, XS,et al. Prevent cervical cancer by screening with reliable human papillomavirus detection and genotyping[J]. CANCER MEDICINE,2012,1(1):59-67.
APA	Ge, SC.,Gong, B.,Cai, XS.,Yang, XE.,Gan, XW.,...&Hong, GF.(2012).Prevent cervical cancer by screening with reliable human papillomavirus detection and genotyping.CANCER MEDICINE,1(1),59-67.
MLA	Ge, SC,et al."Prevent cervical cancer by screening with reliable human papillomavirus detection and genotyping".CANCER MEDICINE 1.1(2012):59-67.