Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation

	Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation
	Liu, YT; Hu, WC; Murakawa, Y; Yin, JW; Wang, G; Landthaler, M; Yan, J
刊名	SCIENTIFIC REPORTS
	2013
卷号	3 期号:1 页码:2054-2054
通讯作者	Yan, J (reprint author), Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Shanghai 200031, Peoples R China.,junyan@picb.ac.cn
英文摘要	The body temperature is considered a universal cue by which the master clock synchronizes the peripheral clocks in mammals, but the mechanism is not fully understood. Here we identified two cold-induced RNA-binding proteins (RBPs), Cirbp and Rbm3, as important regulators for the temperature entrained circadian gene expression. The depletion of Cirbp or Rbm3 significantly reduced the amplitudes of core circadian genes. PAR-CLIP analyses showed that the 3'UTR binding sites of Cirbp and Rbm3 were significantly enriched near the polyadenylation sites (PASs). Furthermore, the depletion of Cirbp or Rbm3 shortened 3'UTR, whereas low temperature (upregulating Cirbp and Rbm3) lengthened 39UTR. Remarkably, we found that they repressed the usage of proximal PASs by binding to the common 39UTR, and many cases of proximal/ distal PAS selection regulated by them showed strong circadian oscillations. Our results suggested that Cirbp and Rbm3 regulated the circadian gene expression by controlling alternative polyadenylation (APA).
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	3' UNTRANSLATED REGIONS ; MICRORNA TARGET SITES ; INDIVIDUAL FIBROBLASTS ; MOLECULAR-MECHANISM ; BODY-TEMPERATURE ; MESSENGER-RNAS ; SHOCK PROTEIN ; WIDE ANALYSIS ; STEM-CELLS ; RHYTHMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000320754200002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/491]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, YT,Hu, WC,Murakawa, Y,et al. Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation[J]. SCIENTIFIC REPORTS,2013,3(1):2054-2054.
APA	Liu, YT.,Hu, WC.,Murakawa, Y.,Yin, JW.,Wang, G.,...&Yan, J.(2013).Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation.SCIENTIFIC REPORTS,3(1),2054-2054.
MLA	Liu, YT,et al."Cold-induced RNA-binding proteins regulate circadian gene expression by controlling alternative polyadenylation".SCIENTIFIC REPORTS 3.1(2013):2054-2054.