Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes

	Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes
	Hong, R; Bai, WY; Zhai, JW; Liu, W; Li, XY; Zhang, JM; Cui, XX; Zhao, X; Ye, XL; Deng, Q
刊名	JOURNAL OF VIROLOGY
	2013
卷号	87 期号:12 页码:6615-6624
通讯作者	Liu, J (reprint author), Fudan Univ, Key Lab Med Mol Virol, Minist Hlth, Shanghai 200433, Peoples R China.,liujing212@fudan.edu.cn ; yhxie@fudan.edu.cn
英文摘要	Hepatitis B virus (HBV) has extremely restricted host and hepatocyte tropism. HBV-based vectors could form the basis of novel therapies for chronic hepatitis B and other liver diseases and would also be invaluable for the study of HBV infection. Previous attempts at developing HBV-based vectors encountered low yields of recombinant viruses and/or lack of sufficient infectivity/cargo gene expression in primary hepatocytes, which hampered follow-up applications. In this work, we constructed a novel vector based on a naturally occurring, highly replicative HBV mutant with a 207-bp deletion in the preS1/polymerase spacer region. By applying a novel insertion strategy that preserves the continuity of the polymerase open reading frame (ORF), recombinant HBV (rHBV) carrying protein or small interfering RNA (siRNA) genes were obtained that replicated and were packaged efficiently in cultured hepatocytes. We demonstrated that rHBV expressing a fluorescent reporter (DsRed) is highly infective in primary tree shrew hepatocytes, and rHBV expressing HBV-targeting siRNA successfully inhibited antigen expression from coinfected wild-type HBV. This novel HBV vector will be a powerful tool for hepatocyte-targeting gene delivery, as well as the study of HBV infection.
学科主题	Virology
类目[WOS]	Virology
关键词[WOS]	CHRONIC HBV INFECTION ; IN-VITRO ; REVERSE-TRANSCRIPTASE ; EXPRESSION ; CELLS ; REPLICATION ; DOMAINS ; BINDING ; GENOME ; LENGTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000319508600009
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/451]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Hong, R,Bai, WY,Zhai, JW,et al. Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes[J]. JOURNAL OF VIROLOGY,2013,87(12):6615-6624.
APA	Hong, R.,Bai, WY.,Zhai, JW.,Liu, W.,Li, XY.,...&Xie, YH.(2013).Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes.JOURNAL OF VIROLOGY,87(12),6615-6624.
MLA	Hong, R,et al."Novel Recombinant Hepatitis B Virus Vectors Efficiently Deliver Protein and RNA Encoding Genes into Primary Hepatocytes".JOURNAL OF VIROLOGY 87.12(2013):6615-6624.