Cancer targeting gene-viro-therapy for pancreatic cancer using oncolytic adenovirus ZD55-IL-24 in immune-competent mice

	Cancer targeting gene-viro-therapy for pancreatic cancer using oncolytic adenovirus ZD55-IL-24 in immune-competent mice
	He, B; Huang, XY; Liu, XY; Xu, B
刊名	MOLECULAR BIOLOGY REPORTS
	2013
卷号	40 期号:9 页码:5397-5405
关键词	CTGVT Oncolytic adenovirus ZD55-IL-24 Pancreatic cancer Immune-competent
通讯作者	He, B (reprint author), Shanghai Jiaotong Univ Sch Med, Xinhua Hosp, Dept Anesthesiol, Kongjiang Rd 1665, Shanghai 200092, Peoples R China.,15000330960@139.com ; pfdbs1@yahoo.com.cn
英文摘要	Cancer targeting gene-viro-therapy (CTGVT) may prove to be an effective treatment for pancreatic cancer (PC). This study was intended to explore the anti-tumor effect of ZD55-IL-24 (oncolytic adenovirus ZD55 harboring IL-24) on PC in immune-competent mice. The expression of gene harbored by oncolytic adenovirus ZD55 in PC cells was detected by reporter-gene assays. The in vitro anti PC ability of ZD55-IL-24 was tested by MTT, crystal violet staining and apoptosis assays. The in vivo anti PC effect of ZD55-IL-24 was further observed in an immune-competent mice model by detecting anti-tumor immunity and induction of apoptosis. The expression of gene harbored by ZD55 in PC cells was significantly higher than that harbored by the replicated-deficient adenovirus, and the amount of gene expression was time-dependent and dose-dependent. Both ZD55-IL-24 and ZD55 inhibited PC cells growth, but the anti-tumor effect of ZD55-IL-24 was significantly stronger than that of ZD55, and the ability of ZD55-IL-24 in inducing PC apoptosis was significantly stronger than that of ZD55. The tumor-forming rate of group ZD55-IL-24 was the lowest, and the tumor-growing rate was also significantly lower than that of group ZD55 in immune-competent PC models. Moreover, ZD55-IL-24 mediated more anti-cancer immunity effects by induction of stronger T-lymphocytes response to PC cells, higher levels of gamma-IFN and IL-6 cytokines. ZD55-IL-24-mediated CTGVT could inhibit PC growth not only by inducing oncolysis and apoptosis but enhancing the anti-cancer immune effects by inducing T cell response to PC and up-regulating gamma-IFN and IL-6 cytokine in immune-competent mice. This may serve as a candidate therapeutic approach for the treatment of PC.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	ANTITUMOR-ACTIVITY ; TUMOR-CELLS ; MDA-7/IL-24 ; VIROTHERAPY ; INDUCTION ; INJECTION ; APOPTOSIS ; INGN-241
收录类别	SCI
语种	英语
WOS记录号	WOS:000323504000018
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/427]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	He, B,Huang, XY,Liu, XY,et al. Cancer targeting gene-viro-therapy for pancreatic cancer using oncolytic adenovirus ZD55-IL-24 in immune-competent mice[J]. MOLECULAR BIOLOGY REPORTS,2013,40(9):5397-5405.
APA	He, B,Huang, XY,Liu, XY,&Xu, B.(2013).Cancer targeting gene-viro-therapy for pancreatic cancer using oncolytic adenovirus ZD55-IL-24 in immune-competent mice.MOLECULAR BIOLOGY REPORTS,40(9),5397-5405.
MLA	He, B,et al."Cancer targeting gene-viro-therapy for pancreatic cancer using oncolytic adenovirus ZD55-IL-24 in immune-competent mice".MOLECULAR BIOLOGY REPORTS 40.9(2013):5397-5405.