Proteome identification of proteins interacting with histone methyltransferase SET8

	Proteome identification of proteins interacting with histone methyltransferase SET8
	Qin, Y; Ouyang, HF; Liu, J; Xie, YH
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2013
卷号	45 期号:4 页码:303-308
关键词	SET8 H4K20me1 mass spectrometry DDX21 rRNA
通讯作者	Xie, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,yhxie@fudan.edu.cn
英文摘要	SET8 (also known as PR-Set7/9, SETD8, KMT5A), a member of the SET domain containing methyltransferase family, which specifically catalyzes mono-methylation of K20 on histone H4 (H4K20me1), has been implicated in multiple biological processes, such as gene transcriptional regulation, cell cycle control, genomic integrity maintenance and development. In this study, we used GST-SET8 fusion protein as bait to search for SET8 interaction partners to elucidate physiological functions of SET8. In combination with mass spectrometry, we identified 40 proteins that potentially interact with SET8. DDX21, a nucleolar protein, was further confirmed to associate with SET8. Furthermore, we discovered a novel function of SET8 in the regulation of rRNA transcription.
学科主题	Biochemistry & Molecular Biology; Biophysics
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	S-PHASE ; PR-SET7 ; PCNA ; H4 ; MONOMETHYLATION ; METHYLATION ; PROGRESSION ; CHROMATIN ; GENES ; TRANSCRIPTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000317610100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/378]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Qin, Y,Ouyang, HF,Liu, J,et al. Proteome identification of proteins interacting with histone methyltransferase SET8[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2013,45(4):303-308.
APA	Qin, Y,Ouyang, HF,Liu, J,&Xie, YH.(2013).Proteome identification of proteins interacting with histone methyltransferase SET8.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,45(4),303-308.
MLA	Qin, Y,et al."Proteome identification of proteins interacting with histone methyltransferase SET8".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 45.4(2013):303-308.