Genome-wide Profiling of 5-Formylcytosine Reveals Its Roles in Epigenetic Priming

	Genome-wide Profiling of 5-Formylcytosine Reveals Its Roles in Epigenetic Priming
	Song, CX; Szulwach, KE; Dai, Q; Fu, Y; Mao, SQ; Lin, L; Street, C; Li, YJ; Poidevin, M; Wu, H
刊名	CELL
	2013
卷号	153 期号:3 页码:678-691
通讯作者	Jin, P (reprint author), Emory Univ, Sch Med, Dept Human Genet, 615 Michael St, Atlanta, GA 30322 USA.,jin@emory.edu ; chuanhe@uchicago.edu
英文摘要	TET proteins oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC). 5fC and 5caC are excised by mammalian DNA glycosylase TDG, implicating 5mC oxidation in DNA demethylation. Here, we show that the genomic locations of 5fC can be determined by coupling chemical reduction with biotin tagging. Genome-wide mapping of 5fC in mouse embryonic stem cells (mESCs) reveals that 5fC preferentially occurs at poised enhancers among other gene regulatory elements. Application to Tdg null mESCs further suggests that 5fC production coordinates with p300 in remodeling epigenetic states of enhancers. This process, which is not influenced by 5hmC, appears to be associated with further oxidation of 5hmC and commitment to demethylation through 5fC. Finally, we resolved 5fC at base resolution by hydroxylamine-based protection from bisulfite-mediated deamination, thereby confirming sites of 5fC accumulation. Our results reveal roles of active 5mC/5hmC oxidation and TDG-mediated demethylation in epigenetic tuning at regulatory elements.
学科主题	Biochemistry & Molecular Biology; Cell Biology
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	EMBRYONIC STEM-CELLS ; THYMINE DNA GLYCOSYLASE ; BASE-RESOLUTION ; MAMMALIAN DNA ; TET PROTEINS ; SELF-RENEWAL ; 5-HYDROXYMETHYLCYTOSINE ; 5-METHYLCYTOSINE ; DEMETHYLATION ; METHYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000318063500019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/358]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Song, CX,Szulwach, KE,Dai, Q,et al. Genome-wide Profiling of 5-Formylcytosine Reveals Its Roles in Epigenetic Priming[J]. CELL,2013,153(3):678-691.
APA	Song, CX.,Szulwach, KE.,Dai, Q.,Fu, Y.,Mao, SQ.,...&He, C.(2013).Genome-wide Profiling of 5-Formylcytosine Reveals Its Roles in Epigenetic Priming.CELL,153(3),678-691.
MLA	Song, CX,et al."Genome-wide Profiling of 5-Formylcytosine Reveals Its Roles in Epigenetic Priming".CELL 153.3(2013):678-691.